Product Information

Contents: Affinity Purified anti-human Toll-like receptor 6 (TLR6, TLR-6)
Catalog Number: 14-9069
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: hPer6
Isotype: Rat IgG2a, κ

Staining of MEG-01 cells with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified hPer6 (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813) and Streptavidin-PE (cat. 12-4317) . Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9069	Affinity Purified anti-human Toll-like receptor 6 (TLR6, TLR-6)	N/A	N/A	FC WB
13-9069	Biotin anti-human Toll-like receptor 6 (TLR6, TLR-6)	N/A	N/A	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The hPer6 antibody was generated against a recombinant protein fragment of human TLR6. To date, at least twelve members of the Toll Like Receptor family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. TLR6 is reported to be expressed in spleen, thymus, ovary and lung and to associate with TLR2.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This hPer6 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB).

Applications Tested

This hPer6 antibody has been tested by flow cytometric analysis of human MEG-01 cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Bulut Y, Faure E, Thomas L, Equils O, Arditi M. 2001. Cooperation of Toll-like receptor 2 and 6 for cellular activation by soluble tuberculosis factor and Borrelia burgdorferi outer surface protein A lipoprotein: role of Toll-interacting protein and IL-1 receptor signaling molecules in Toll-like receptor 2 signaling. J Immunol. 167(2): 987-94.
Takeuchi O, Kawai T, Sanjo H, Copeland NG, Gilbert DJ, Jenkins NA, Takeda K, Akira S. 1999. TLR6: A novel member of an expanding toll-like receptor family. Gene. 231(1-2): 59-65.

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