Immunoblot analysis of reduced human heart cell lysates using Anti-TIRAP Purified at 0.5 (lane A), 1 (Lane B), and 2 (Lane C) µg/ml and detected using Anti-Rabbit IgG-HRP.
Contents: Anti-TIRAP Purified Catalog Number: 14-9058 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Clone: Polyclonal Host/Isotype: Rabbit IgG
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14-9058-63
10 ug
14-9058-81
50 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The polyclonal antibody reacts with human, mouse, and rat Toll-interleukin 1 receptor (TIR) domain-containing adapter protein, TIRAP, and was generated using 15 amino acid residues near the C-terminus of mouse TIRAP. TIRAP, a signaling adapter molecule also known as myeloid differentiation protein 88 adapter-like (Mal) is involved in the signaling pathway of TLR4. TIRAP-deficient mice respond normally to the TLR5, TLR7 and TLR9 ligands, as well as to IL-1 and IL-18, but have defects in cytokine production and in activation of the nuclear factor NF-kappaB and mitogen-activated protein kinases in response to lipopolysaccharide, a ligand for TLR4. In addition, TIRAP-deficient mice are also impaired in their responses to ligands for TLR2, TLR1 and TLR6. Immunoblotting with this antibody gives a MW of ~35kDa in human heart lysate.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Rabbit anti-human, mouse, rat TIRAP poly has been reported for use in immunoblotting (WB).
Applications Tested
Rabbit anti-human, mouse, rat TIRAP poly has been tested by immunoblotting (WB) of human heart lysate (1-2ug/ml). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Horng T, Barton GM, Medzhitov R. 2001. TIRAP: an adapter molecule in the Toll signaling pathway.
Nat Immunol. 2(9):835-41. Henneke P, Golenbock DT. 2001. TIRAP: how Toll receptors fraternize. Nat Immunol. 2(9):828-30. Shinobu N, Iwamura T, Yoneyama M, Yamaguchi K, Suhara W, Fukuhara Y, Amano F, Fujita T. 2002. Involvement of TIRAP/MAL in signaling for the activation of interferon regulatory factor 3 by lipopolysaccharide. FEBS Lett. 517(1-3):251-6.
