Product Information

Contents: Affinity Purified anti-human Toll-like receptor 3 (CD283, TLR3, TLR-3)
Catalog Number: 14-9039
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: TLR3.7
Isotype: Mouse IgG1, κ

Staining of permeabilized A549 cells with 1 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 1 μg of Purified anti-human TLR3 (TLR3.7) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-9039	PE anti-human Toll-like receptor 3 (CD283, TLR3, TLR-3)	488	575	IC Flow
14-9039	Affinity Purified anti-human Toll-like receptor 3 (CD283, TLR3, TLR-3)	N/A	N/A	IC Flow IH/F IHC(Paraffin) IP WB
16-9039	Functional Grade* Purified anti-human Toll-like receptor 3 (CD283, TLR3, TLR-3)	N/A	N/A	FA IC Flow
13-9039	Biotin anti-human Toll-like receptor 3 (CD283, TLR3, TLR-3)	N/A	N/A	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The TLR3.7 monoclonal antibody reacts with human Toll-like receptor 3 (TLR3). To date, at least twelve members of the Toll-like receptor family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. In the innate immune response, TLRs recognize molecular patterns associated with microbial pathogens and induce antimicrobial activity. TLR3 recognizes double-stranded (ds)RNA, induces the activation of NF-κB through MyD88-dependent and -independent pathways, and the production of type I interferons (IFNs). TLR3.7 suppressed poly(I):poly(C)-mediated IFN-β production by human fibroblasts naturally expressing TLR3 on their surface.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This TLR3.7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections. (Fluorochrome conjugated TLR3.7 is recommended for use in intracellular flow cytometry.) It has also been reported in in vitro inhibition of ligand binding. For optimal detection of TLR3 with this mAb by flow cytometry, PE-conjugated or Biotin-conjugated TLR3.7 (revealed with SAv-PE cat# 12-4312) are recommended.

Applications Tested

This TLR3.7 antibody has been tested by intracellular staining and flow cytometric analysis of A549 and MRC5 cell lines to detect the very low level of this antigen on the surface. This can be used at less than or equal to 2.0 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

\Matsumoto M, Kikkawa S, Kohase M, Miyake K, Seya T. 2002. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem Biophys Res Commun. 293:1364-9

Oshiumi H, Matsumoto M, Funami K, Akazawa T, Seya T. 2003. TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction. Nat Immunol. 4:161-7

Alexopoulou L, Holt AC, Medzhitov R, Flavell RA. 2001. Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature. 413:732-8

Jorgenson RL, Lesmeister MJ et al. 2005. Human endometrial epithelial cells cyclically express toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA. Hum Immunol 66(5):469-482. (IHC paraffin and IHC frozen, PubMed)

Schreiner B, Voss J et al. 2005. Expression of toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release and up-regulation of NKG2D-ligands. FASEB J 20(1):118-20. (IHC frozen and WB, PubMed)

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