Product Information

Contents: Affinity Purified anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)
Catalog Number: 14-9022
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: mT2.7
Isotype: Mouse IgG2a, κ

Staining of Raw246.7 cells with 0.25 μg oPurified Mouse IgG2a, K Iso Cntrl (cat. 14-4724) (open histogram) or 0.25 μg Purified anti-mouse TLR2 (mT2.7)(colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013) and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9022	Affinity Purified anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC IH/F IP
13-9022	Biotin anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC
11-9022	Fluorescein isothiocyanate (FITC) anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	518	FC
12-9022	Phycoerythrin (PE) anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	575	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The mT2.7 monoclonal antibody reacts with mouse Toll-like receptor 2 (TLR2). Mouse TLR2 is expressed by the myeloid lineage, including macrophage and dendritic cells in splenocytes and the RAW264.7 cell line. To date, at least twelve members of the Toll family have been identified in human and mouse. This family of type I transmembrane protein is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed on the surface of cells and is responsible for distinguishing different pathogens.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This mT2.7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The mT2.7 antibody has been tested by flow cytometric analysis of Raw246.7 cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Meng G, Rutz M, Schiemann M, Metzger J, Grabiec A, Schwandner R, Luppa PB, Ebel F, Busch DH, Bauer S, Wagner H, Kirschning CJ. 2004. Antagonistic antibody prevents toll-like receptor 2-driven lethal shock-like syndromes. J Clin Invest. 2004. 113:1473-81.

Related Products

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4724    Affinity Purified Mouse IgG2a, K Isotype Control
Cat. 14-8185    Recombinant B18R protein (Vaccinia Virus-Encoded Neutralizing Type I Interferon Receptor; Type I IFN inhibitor)
Cat. 34-8185    Carrier-Free Recombinant B18R protein (Vaccinia Virus-Encoded Neutralizing Type I Interferon Receptor; Type I IFN inhibitor)
Cat. 11-8317    Fluorescein isothiocyanate (FITC) mouse anti-human IFN-alpha2 (clone 225.C)
Cat. 12-8317    Phycoerythrin (PE) mouse anti-human IFN-alpha2 (clone 225.C)
Cat. 17-8317    Allophycocyanin (APC) mouse anti-human IFN-alpha2 (clone 225.C)
Cat. 11-9021    FITC anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2) (clone 6C2)
Cat. 12-9021    PE anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2) (clone 6C2)
Cat. 13-9021    Biotin anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2) (clone 6C2)
Cat. 14-9021    Affinity Purified anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2) (clone 6C2)
Cat. 16-9021    Functional Grade Purified anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2) (clone 6C2)
Cat. 12-9024    Phycoerythrin (PE) anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2) (clone T2.5)
Cat. 13-9024    Biotin anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2) (clone T2.5)
Cat. 14-9024    Affinity Purified anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2) (clone T2.5)
Cat. 16-9024    Functional Grade Purified anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2) (clone T2.5)