human Fn14 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-human Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14)
Catalog Number: 14-9019
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: ITEM-1
Isotype: Mouse IgG1, κ

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14-9019-80

25 ug

14-9019-82

100 ug

Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 1.0 µg of purified mouse IgG1 isotype control (cat.11-4719) (open histogram) or 0.5 µg of purified ITEM-1 (colored histogram) followed by FITC anti-mouse IgG (cat.11-4011). Total viable cells were analyzed.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9019	Affinity Purified anti-human Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14)	N/A	N/A	FA FC IH/F
12-9019	PE anti-human Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The ITEM-1 mAb reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The ITEM-1 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. It has also been reported in induction of cell death in transfected cells. (Please use Functional Grade purified ITEM-1 in functional assays.)

Applications Tested

The ITEM-1 antibody has been tested by flow cytometric analysis on human Fn14 transfected cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nakayama, M., K. Ishidoh, Y. Kojima, N. Harada, E. Kominami, K. Okumura, H. Yagita. 2003. Fibroblast Growth Factor-Inducible 14 Mediates Multiple Pathways of TWEAK-Induced Cell Death. The Journal of Immunology, 170: 341-348.
Harada N., M. Nakayama, H. Nakano, Y. Fukuchi, H. Yagita, K. Okumura. 2002. Pro-inflammatory Effect of TWEAK/Fn14 Interaction on Human Umbilical Vein Endothelial Cells. Biochem Biophys Res Commun, 299(3):488-93.
Nakayama M, K. Ishidoh, N. Kayagaki, Y. Kojima, N. Yamaguchi, H. Nakano, E. Kominami, K. Okumura, H. Yagita. 2002. Multiple pathways of TWEAK-induced cell death. J Immunol, 168(2):734-43

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