mouse/human Fn14 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-human/mouse Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14)
Catalog Number: 14-9016
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: ITEM-2
Isotype: Mouse IgA, κ

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14-9016-80

25 ug

14-9016-82

100 ug

Staining of human Fn14-transfected L5178Y cells with 0.06 μg of Purified Mouse IgA Iso Cntrl (cat. 14-4762) (open histogram) or 0.06 μg of Purified anti-hu/mo Fn14 (ITEM-2) (colored histogram) followed by FITC anti-mouse IgA (cat. 11-4204). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9016	Affinity Purified anti-human and mouse Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14)	N/A	N/A	FA FC
16-9016	Functional Grade* Purified anti-human and mouse Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The ITEM-2 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducable 14 kDa protein) and cross-reacts with the mouse antigen. Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1 (Cat. No. 16-9915). Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line. ITEM-2 is reported to act as an antagonist in in vitro functional assays.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The ITEM-2 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro blocking of mouse/human Fn14. (Please use Functional Grade purified ITEM-2, cat. 16-9016, in functional assays.)

Applications Tested

The ITEM-2 antibody has been tested by flow cytometric analysis of human Fn14 transfected cells. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nakayama, M., K. Ishidoh, Y. Kojima, N. Harada, E. Kominami, K. Okumura, H. Yagita. 2003. Fibroblast Growth Factor-Inducible 14 Mediates Multiple Pathways of TWEAK-Induced Cell Death. The Journal of Immunology, 170: 341-348.
Harada N., M. Nakayama, H. Nakano, Y. Fukuchi, H. Yagita, K. Okumura. 2002. Pro-inflammatory Effect of TWEAK/Fn14 Interaction on Human Umbilical Vein Endothelial Cells. Biochem Biophys Res Commun, 299(3):488-93.
Nakayama M, K. Ishidoh, N. Kayagaki, Y. Kojima, N. Yamaguchi, H. Nakano, E. Kominami, K. Okumura, H. Yagita. 2002. Multiple pathways of TWEAK-induced cell death. J Immunol, 168(2):734-43

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