Product Information

Contents: Affinity Purified anti-mouse Toll-like receptor 1 (CD281, TLR1, TLR-1)
Catalog Number: 14-9012
Sizes: 25 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioLD5
Isotype: Rat IgG2a

Left Panel: Untransfected control (left) and mouse TLR1-transfected (right) Baf/3 cell lysates were loaded at 1x10⁵ cells/lane, probed with 3 µg/mL purified eBioLD5 and revealed with HRP anti-rat IgG.

Right Panel: Peritoneal-exudate (left) and RAW264.7 (right) cell lysates were loaded at 1x10⁵ cells/lane, probed with 3 μg/ml purified eBioLD5 and revealed with HRP anti-rat IgG.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9012	Affinity Purified anti-mouse Toll-like receptor 1 (CD281, TLR1, TLR-1)	N/A	N/A	WB

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Description

The eBioLD5 monoclonal antibody reacts with mouse Toll-like receptor 1 (TLR1, CD281). TLR1 is an approximately 98 kDa protein belonging to the Toll-like receptor family, and is closely-related to mouse TLR6 in both structure and function. Through the formation of a complex with TLR2, TLR1 is able to bind bacterially-derived triacyl lipopeptides, as well as soluble factors released from Neisseria meningitidis. TLR1 is expressed by macrophages and macrophages in TLR1-deficient mice display impaired production of inflammatory cytokines upon stimulation with triacyl lipopeptides and lipoproteins from mycobacteria.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioLD5 antibody has been reported for use in immunoblotting (WB).

Applications Tested

This eBioLD5 antibody has been tested by immunoblotting of mouse TLR1-transfected, peritoneal-exudate, and RAW264.7 cells. This can be used at 1-3 μg/ml for western blotting analysis. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Alexopoulou L, Thomas V, Schnare M, Lobet Y, Anguita J, Schoen RT, Medzhitov R, Fikrig E, Flavell RA. Hyporesponsiveness to vaccination with Borrelia burgdorferi OspA in humans and in TLR1- and TLR2-deficient mice. Nat Med. 2002 Aug;8(8):878-84.

Wyllie DH, Kiss-Toth E, Visintin A, Smith SC, Boussouf S, Segal DM, Duff GW, Dower SK. Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterial responses. J Immunol. 2000 Dec 15;165(12):7125-32.

Takeuchi O, Sato S, Horiuchi T, Hoshino K, Takeda K, Dong Z, Modlin RL, Akira S. Cutting edge: role of Toll-like receptor 1 in mediating immune response to microbial lipoproteins. J Immunol. 2002 Jul 1;169(1):10-4.

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