Product Information

Contents: Affinity Purified anti-mouse Toll-like receptor 1 (CD281, TLR1, TLR-1)
Catalog Number: 14-9011
Sizes: 25 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioTR23 (TR23)
Isotype: Rat IgG2a, κ

Staining of mouse TLR1-transfected Baf/3 cells with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified anti-mouse TLR1 (eBioTR23) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9011	Affinity Purified anti-mouse Toll-like receptor 1 (CD281, TLR1, TLR-1)	N/A	N/A	FC IP
13-9011	Biotin anti-mouse Toll-like receptor 1 (CD281, TLR1, TLR-1)	N/A	N/A	FC
12-9011	Phycoerythrin (PE) anti-mouse Toll-like receptor 1 (CD281, TLR1, TLR-1)	488	575	FC
51-9011	Alexa Fluor® 647 anti-mouse Toll-like receptor 1 (CD281, TLR1, TLR-1)	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioTR23 monoclonal antibody reacts with mouse Toll-like receptor 1 (TLR1, CD281). TLR1 is an approximately 98 kDa protein belonging to the Toll-like receptor family, and is closely-related to mouse TLR6 in both structure and function. Through the formation of a complex with TLR2, TLR1 is able to bind bacterially-derived triacyl lipopeptides, as well as soluble factors released from Neisseria meningitidis. TLR1 is expressed by macrophages and macrophages in TLR1-deficient mice display impaired production of inflammatory cytokines upon stimulation with triacyl lipopeptides and lipoproteins from mycobacteria.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioTR23 (TR23) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested

This eBioTR23 (TR23) antibody has been tested by flow cytometric analysis of mouse TLR1-transfected Baf/3 cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Alexopoulou L, Thomas V, Schnare M, Lobet Y, Anguita J, Schoen RT, Medzhitov R, Fikrig E, Flavell RA. Hyporesponsiveness to vaccination with Borrelia burgdorferi OspA in humans and in TLR1- and TLR2-deficient mice. Nat Med. 2002 Aug;8(8):878-84.

Wyllie DH, Kiss-Toth E, Visintin A, Smith SC, Boussouf S, Segal DM, Duff GW, Dower SK. Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterial responses. J Immunol. 2000 Dec 15;165(12):7125-32.

Takeuchi O, Sato S, Horiuchi T, Hoshino K, Takeda K, Dong Z, Modlin RL, Akira S. Cutting edge: role of Toll-like receptor 1 in mediating immune response to microbial lipoproteins. J Immunol. 2002 Jul 1;169(1):10-4.

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