Product Information

Contents: Recombinant mouse FGFb (FGF-basic)
Catalog Number: 14-8982
Sizes: 5 ug, 25 ug
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by mouse BALB/c 3T3 cell proliferation assay. The ED₅₀ is typically 0.1 ng/ml, corresponding to a specific activity of 10⁷ units/mg.

Mouse FGFb bioactivity measured by mouse BALB/c 3T3 cell proliferation assay. Recombinant cytokine concentration in pg/ml.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8982	Recombinant mouse FGFb (FGF-basic)	N/A	N/A	BA

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Description

Mouse Fibroblast Growth Factor-basic (FGF-basic) is a 16 kDa protein derived from such tissues as brain, kidney, adrenal glands, monocytes, and epithelial cells. FGF-basic stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse FGF-basic has been reported for use in cytokine bioassays, and ELISA.

Applications Tested

This reagent has been tested by bioassays using the FGF-basic-responsive cell line BALB/c 3T3. The ED₅₀, as determined by the dose-dependent stimulation of 3T3 cell proliferation is 0.1 ng/ml, corresponding to a specific activity of 10⁷ units/mg.

References

Eckenstein, F. P. et al. 1994. Fibroblast growth factors in the nervous system. J. Neurobiol. 25: 1467-1480.

Jung, J. et al. 1999. Initiation of mammalian liver development from endoderm by fibroblast growth factors. Science 284: 1998-2003.

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