Contents: Human Resistin Recombinant Protein Catalog Number: 14-8956 Concentration: 0.1 mg/mL Formulation: Sterile liquid, phosphate buffered saline with 1% BSA, pH 7.2 Storage Conditions: Store at less than or equal to -70°C. Handling Conditions: For best recovery, quick-spin vial prior to opening. Use in sterile envrioment. Source:E. coli expressed amino acids Ser17-Pro108 accession number NP_065148 Molecular Mass: 9.9 kDa Purity: Greater than 97%, as determined by SDS-PAGE Endotoxin Level: Less than 0.1 ng/µg cytokine as determined by the LAL assay. Bioactivity: The ED50 of this protein, as measured by stimulation of lypolysis in human adipocytes, is 7-30 μg/ml. This corresponds to a specific activity of 143-33 Units/mg.
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14-8956-62
5 ug
14-8956-80
25 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
Resistin is a peptide hormone belonging to the RELM (Resistin-Like Molecule) family that also includes RELMα, RELMβ, and RELMγ. In mice, resistin is secreted in high levels by adipocytes, and overexpression has been linked to insulin resistance and obesity. The link between resistin and glucose metabolism in humans is still unclear, and it has instead been found to play a role in the inflammatory process. The main source of resistin in humans is peripheral blood monocytes, which upregulate expression in response to IL-6 and TNFα. Elevated levels of circulating resistin are associated with chronic inflammatory conditions and cardiovascular disease.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Human Resisitin Recombinant Protein is biologically active.
Applications Tested
The ED50 of this protein, as measured by stimulation of lypolysis in human adipocytes, is 7-30 μg/ml. This corresponds to a specific activity of 143-33 Units/mg.
References
Rabe K, Lehrke M, Parhofer KG, Broedl UC. Adipokines and insulin resistance. Mol Med. 2008 Nov-Dec;14(11-12):741-51.
On YK, Park HK, Hyon MS, Jeon ES. Serum resistin as a biological marker for coronary artery disease and restenosis in type 2 diabetic patients. Circ J. 2007 Jun;71(6):868-73.
Pang SS, Le YY. Role of resistin in inflammation and inflammation-related diseases. Cell Mol Immunol. 2006 Feb;3(1):29-34.
