Product Information

Contents: Affinity Purified anti-human TARP (T Cell Receptor g Chain Alternate Reading Frame Protein)
Catalog Number: 14-8868
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioTP1 (TP1, a.k.a 1F8)
Isotype: Mouse IgG2a

Untreated (left lane) and Vitamin A-treated (right lane) LNCaP cell lysates were loaded at 1x105 cells/lane, probed with 2 µg/mL purified eBioTP1 and revealed with HRP anti-mouse IgG.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8868	Affinity Purified anti-human TARP (T Cell Receptor g Chain Alternate Reading Frame Protein)	N/A	N/A	IHC WB

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Description

The eBioTP1 monoclonal antibody recognizes human T Cell Receptor gamma Chain Alternate Reading Frame Protein (TARP). The TARP gene is embedded in the TCR gamma locus and its transcript originates within an intron just upstream of the J gamma 1.2 segment. TARP, which is 58 amino acids and 7 kDa in size, is localized to the mitochondria and expressed exclusively in normal prostate, and in prostate and breast cancers. Furthermore, the expression of TARP is regulated by androgen in cell lines.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioTP1 (TP1, a.k.a 1F8) antibody has been reported for use in immunoblotting (WB), and immunohistochemical staining.

Applications Tested

This eBioTP1 (TP1, a.k.a 1F8) antibody has been tested by western blot analysis of vitamin A-treated LNCaP cells. This antibody can be used at 2 μg/ml as a starting concentration, however it is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Wolfgang CD, Essand M, Vincent JJ, Lee B, Pastan I. TARP: a nuclear protein expressed in prostate and breast cancer cells derived from an alternate reading frame of the T cell receptor gamma chain locus. Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9437-42.

Maeda H, Nagata S, Wolfgang CD, Bratthauer GL, Bera TK, Pastan I. The T cell receptor gamma chain alternate reading frame protein (TARP), a prostate-specific protein localized in mitochondria. J Biol Chem. 2004 Jun 4;279(23):24561-8. (TP1, mAb development, WB, IHC, PubMed)

Cheng WS, Kraaij R, Nilsson B, van der Weel L, de Ridder CM, Totterman TH, Essand M. A novel TARP-promoter-based adenovirus against hormone-dependent and hormone-refractory prostate cancer. Mol Ther. 2004 Aug;10(2):355-64.

Carlsson B, Totterman TH, Essand M. Generation of cytotoxic T lymphocytes specific for the prostate and breast tissue antigen TARP. Prostate. 2004 Oct 1;61(2):161-70.

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