Affinity Purified anti-human TRA-1-60 (podocalyxin)

Contents: Affinity Purified anti-human TRA-1-60 (anti-human Podocalyxin, glycosylation-specific epitope)
Catalog Number: 14-8863
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: TRA-1-60
Isotype: Mouse IgM

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-8863-80	25 ug	$75
14-8863-82	100 ug	$125

*International customers: Please contact your distributor for region specific pricing.

Staining of 2102Ep human embryonal carcinoma cells with 0.25 μg of Purified Mouse IgM Iso Cntrl (cat. 14-4752) (open histogram) or 0.25 μg of Purified anti-human TRA-1-60 (colored histogram) followed by PE anti-mouse IgM (eB121-15F9) (cat. 12-5890). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8863	Affinity Purified anti-human TRA-1-60 (podocalyxin)	N/A	N/A	FC IHC IP WB
13-8863	Biotin anti-human TRA-1-60 (podocalyxin)	N/A	N/A	FC
12-8863	Phycoerythrin (PE) anti-human TRA-1-60 (podocalyxin)	488	575	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This TRA-1-60 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting, and immunofluorescence.

Applications Tested

This TRA-1-60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. This antibody has also been tested by immunoblotting of 2102Ep cell extracts, yielding a band of approximately 200-250 kDa (reducing and non-reducing conditions). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schopperle WM, DeWolf WC. The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells. 2007 Mar;25(3):723-30. (TRA-1-60, WB, PubMed)

Chin AC, Fong WJ, Goh LT, Philp R, Oh SK, Choo AB. Identification of proteins from feeder conditioned medium that support human embryonic stem cells. J Biotechnol. 2007 Jun 30;130(3):320-8. (TRA-1-60, FC, PubMed)

Badcock G, Pigott C, Goepel J, Andrews PW. The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res. 1999 Sep 15;59(18):4715-9. (TRA-1-60, WB, PubMed)

Andrews PW, Banting G, Damjanov I, Arnaud D, Avner P. Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma. 1984 Winter;3(4):347-61. (PubMed)

Related Products

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