Product Information

Contents: Affinity Purified anti-human c-Met (HGFR)
Catalog Number: 14-8858
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioclone 97
Isotype: Rat IgG1, κ

Staining of human c-Met/GFP-transfected cells with 0.5 μg of Purified Rat IgG1 Iso Cntrl (cat. 14-4301) (left) or 0.5 μg of Purified anti-human c-Met (eBioclone 97) (right) followed by PE Anti-Rat IgG (cat. 12-4822). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8858	Affinity Purified anti-human c-Met (HGFR)	N/A	N/A	FC
13-8858	Biotin anti-human c-Met (HGFR)	N/A	N/A	FC
11-8858	Fluorescein isothiocyanate (FITC) anti-human c-Met (HGFR)	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioclone 97 monoclonal antibody was generated against a human c-Met-Ig fusion protein, and reacts with human c-Met (HGFR)-transfected cells. Human c-Met is a 145 kDa receptor tyrosine kinase (RTK) expressed by epithelial cells of the brain, kidney, liver and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation, and activation of several downstream effectors including the mitogen-activated protein kinases ERK-1 and ERK-2, and PLC-γ. Activation of the c-Met signal transduction pathway leads to mulitple cellular responses including cell motility, scattering, proliferation, survival and angiogenesis. Mutations in human c-Met have been implicated in the development of several malignancies.

The eBioclone 97 monoclonal antibody has been tested by flow cytometric analysis of human c-Met-transfected BAF/3 cells. The low level of expression of c-Met in primary tissue may make flow cytometric detection of the endogenous c-Met protein difficult. Experiments are underway to evaluate use eBioclone 97 for flow cytometric detection of c-Met from primary cells. Please contact eBioscience technical support for further information.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioclone 97 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioclone 97 antibody has been tested by flow cytometric analysis of human c-Met-transfected cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Lin JC, Naujokas M, Zhu H, Nolet S, Park M. Intron-exon structure of the MET gene and cloning of an alternatively-spliced Met isoform reveals frequent exon-skipping of a single large internal exon. Oncogene. 1998 Feb 19;16(7):833-42.

Prat M, Crepaldi T, Pennacchietti S, Bussolino F, Comoglio PM. Agonistic monoclonal antibodies against the Met receptor dissect the biological responses to HGF. J Cell Sci. 1998 Jan;111 ( Pt 2):237-47.

Related Products

Cat. 14-4301  Affinity Purified Rat IgG1 Isotype Control
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)