Product Information

Contents: Affinity Purified anti-mouse/human SSEA-3 (stage-specific embryonic antigen-3)
Catalog Number: 14-8833
Sizes: 25 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioMC-631 (MC-631)
Isotype: Rat IgM

Staining of the 2102Ep cell line with 0.5 μg of Purified Rat IgM Iso Cntrl (cat. 14-4341) (open histogram) or 0.5 μg of Purified anti-human SSEA-3 (MC-631) (colored histogram) followed by PE anti-rat IgM (HIS40) (cat. 12-0990). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8833	Affinity Purified anti-human/mouse SSEA-3 (stage-specific embryonic antigen-3)	N/A	N/A	FC IHC
13-8833	Biotin anti-human/mouse SSEA-3 (stage-specific embryonic antigen-3)	N/A	N/A	FC
12-8833	Phycoerythrin (PE) anti-human/mouse SSEA-3 (stage-specific embryonic antigen-3)	488	575	FC
53-8833	Alexa Fluor® 488 anti-human/mouse SSEA-3 (stage-specific embryonic antigen-3)	488	519	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) and down regulated following differentiation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioMC-631 (MC-631) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested

This eBioMC-631 (MC-631) antibody has been tested by flow cytometric analysis of the 2102Ep cell line. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Andrews PW, Goodfellow PN, Shevinsky LH, Bronson DL, Knowles BB. Cell-surface antigens of a clonal human embryonal carcinoma cell line: morphological and antigenic differentiation in culture. Int J Cancer. 1982 May 15;29(5):523-31.

Kannagi R, Cochran NA, Ishigami F, Hakomori S, Andrews PW, Knowles BB, Solter D. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J. 1983;2(12):2355-61.

Related Products

Cat. 11-0990  FITC anti-rat IgM (clone HIS40)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4341  Affinity Purified Rat IgM Isotype Control