Product Information

Contents: Recombinant Human Flt-3/Flk-2 Ligand
Catalog Number: 14-8358
Sizes: 5 ug, 25 ug
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Molecular Mass: Recombinant human Flt3-Ligand is a soluble 17.6 kDa protein consisting of 155 amino acid residues.
Purity: Greater than 98%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 10⁶ units/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8358	Recombinant Human Flt-3/Flk-2 Ligand (Flt3/Flk2 Ligand, Flt3L, Flt3-L, Flk2L, Flk2-L)	N/A	N/A	BA
34-8358	Carrier-Free Recombinant Human Flt-3/Flk-2 Ligand (Flt3/Flk2 Ligand, Flt3L, Flt3-L, Flk2L, Flk2-L)	N/A	N/A	BA

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Description

Flt3-Ligand is a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand exerts no activity on mast cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 a.a.). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform. Recombinant human Flt3-Ligand is a soluble 17.6 kDa protein consisting of 155 amino acid residues.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The recombinant human FLT3 Ligand has been reported for use in bioassay.

Applications Tested

The recombinant human Flt-3 Ligand has been tested for its ability to induce cell proliferation in a bioassay. The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 10⁶ units/mg.

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