Recombinant Human Transforming Growth Factor-β1 (TGF-β1)
RUO: For Research Use Only
Contents: Recombinant Human Transforming Growth Factor-β1 (TGF-β1) Catalog Number: 14-8348 Formulation: Sterile liquid; 0.1 M Gly-HCl, pH3.0. 0.22 uM filtered, 0.1% BSA as a stabilizer. Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles. Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent. Source: Human TGFb1 was expressed in CHO cells. Mature TGFb1 (ala279 - ser390; accession #BC001180) was purified from the conditioned media. Molecular Mass: Mature TGFb1 polypeptide has a predicted molecular mass of 12,794. On reducing SDS-PAGE the protein migrates as a 13 kDa polypeptide and on non-reducing SDS page the cystine-linked homodimer migrates as a 25 kDa protein. Purity: Greater than 98%, as determined by SDS-PAGE Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Bioactivity: Measured by inhibition of IL-4 induced mouse HT-2 cell proliferation. The ED50 is 0.30 ng/ml, corresponding to a specific activity of 3 x 106 Units/mg.
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14-8348-62
5 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
Human TGF-β1 (Transforming Growth Factor-β1) is a member of a superfamily of homologous, disulfide-linked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. Human TGF-β1 is a 25 kDa protein with each subunit containing 112 aa residues.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant human TGF-β1 is biologically active and can inhibit IL-4 induced proliferation of mouse HT-2 cells. Recombinant human TGF-β1 is useful as an ELISA standard.
Applications Tested
This recombinant human TGF-β1 has been tested in bioassay for inhibition of IL-4-dependent proliferation of mouse HT-2 cells. The ED50 measured in such assays is typically < 0.30 ng/ml, corresponding to a bioactivity of > 3 x 106 units/mg.
References
Kotenko, S., et al. 2003. IFN-lambdas mediate anti-viral protection through a distinct class II cytokine receptor complex. Nature Immunol. 4: 69-77.
Mennechet, F., et al. 2006. IFN-lambda-treated dendritic cells specifically induce proliferation of FOXP3-expressing suppressor T cells. Immunobiology. 107: 4417-4423.
Sheppard, P., et al. 2003. IL-28, IL-29, and their class II cytokine receptor IL-28R. Nature Immunol. 4: 63-68.
