Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF)
RUO: For Research Use Only
Contents: Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Catalog Number: 14-8339 Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered. Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles. Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent. Source: E. coli expressed amino acids ala 18 - glu144 of mature human GMCSF (accession # NM_000758). Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 14,478. The DTT reduced protein migrates as a 14 kDa polypeptide on SDS-PAGE. The non-reduced protein migrates as a 13 kDa polypeptide. Purity: Greater than 98%, as determined by SDS-PAGE Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Bioactivity: Measured by human TF-1 cell proliferation assay. The ED50 is 0.1 ng/ml, corresponding to a specific activity of 1.0 x 107 Units/mg.
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14-8339-62
5 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
Human GM-CSF (Granulocyte/Monocyte-Colony Stimulating Factor) is a differentially glycosylated factor produced mainly by activated T cells and macrophages. Endothelial cells and fibroblasts can also produce GM-CSF after exposure to TNF-α, IL-1, IL-2 and IFN-γ. GM-CSF is found associated with extracellular matrix and in membrane-bound formats too. GM-CSF stimulates proliferation, activation and differentiation of macrophages and granulocytes and their progenitors.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant human GM-CSF is biologically active and can promote proliferation of human TF-1 cell line in culture. It is also used as a standard for a human GM-CSF ELISA.
Applications Tested
This recombinant human GM-CSF has been tested in bioassays using human TF-1 cell line. The ED50 measured in a TF-1 proliferation assay is typically 100 pg/ml, corresponding to a specific activity of approximately 1.0 x107 Units/mg.
