Product Information

Contents: Recombinant mouse IL-33, Interleukin 33
Catalog Number: 14-8332
Sizes: 5 ug, 25 ug
Formulation: Sterile liquid; 10 mM Phosphate, 150 mM NaCl, 2 mM DTT, pH 7.2 (PBS with DTT) with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed protein consisting of amino acids Ser 109-Ile 266 of mature mouse IL-33 (accession # AK075849).
Molecular Mass: The DTT reduced protein migrates as a 15 kDa polypeptide on SDS-PAGE.
Purity: Greater than 97%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by D10.G4.1 cell proliferation assay. The ED50 is 0.03 ng/ml, corresponding to a specific activity of 3.0 x 107 Units/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8332	Recombinant mouse IL-33, Interleukin 33	N/A	N/A	BA ELISA std
34-8332	Carrier-Free Recombinant mouse IL-33, Interleukin 33	N/A	N/A	BA ELISA std
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

Mouse IL-33, also called NF-HEV and DVS 27, is a 30 kDa proinflammatory cytokine produced by endothelial and epithelial cells. IL-33 is released during necrotic cell death but was first found in the nucleus of endothelial cells. This dual pattern of expression is reminiscent of "alarmins" also known as endogeneous danger signals. Other known alarmins are IL-1α and HMGB1. IL-33 has been identified as the ligand for ST2 (a member of the Il-1 receptor family). ST2 is stably expressed on mast cells and T(h)2 effector T cells and is functionally associated with T(h)2-mediated inflammation. Although IL-33 was initially reported to be processed by Caspase-1, recent data indicate that Caspase-3 or -7 are capable of processing IL-33 at more physiologic concentrations. IL-33 plays an immune regulatory role by inducing IL-5 and IL-13 in vitro and in vivo and activating basophils, eosinophils and mast cells. In addition to its role in proimflammation, it may also decrease inflammation through interactions with IL-1 thereby blocking its effect. Mouse IL-33 share 55% amino acid sequence identity with human and 90% with rat.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified Recombinant mouse IL-33 has been reported for use in cytokine bioassays.

Applications Tested

This recombinant mouse IL-33 has been tested by bioassays using the cell line D10.G4.1. The ED50 measured in the D10.G4.1 proliferation assay is typically less than 0.1 ng/ml, corresponding to a specific activity of greater than 1x107 Units/mg.

References

Lüthi AU, Cullen SP, McNeela EA, Duriez PJ, Afonina IS, Sheridan C, Brumatti G, Taylor RC, Kersse K, Vandenabeele P, Lavelle EC, Martin SJ. Suppression of interleukin-33 bioactivity through proteolysis by apoptotic caspases. Immunity. 2009 Jul 17;31(1):84-98.

Verri WA Jr, Guerrero AT, Fukada SY, Valerio DA, Cunha TM, Xu D, Ferreira SH, Liew FY, Cunha FQ. IL-33 mediates antigen-induced cutaneous and articular hypernociception in mice. Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2723-8. Epub 2008 Feb 4.

Schmitz J, Owyang A, Oldham E, Song Y, Murphy E, McClanahan TK, Zurawski G, Moshrefi M, Qin J, Li X, Gorman DM, Bazan JF, Kastelein RA. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity. 2005 Nov;23(5):479-90.