Product Information

Contents: Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF)
Catalog Number: 14-8331
Sizes: 5 ug, 25 ug
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, 1% BSA, 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed mature mouse GM-CSF (accession # NM_009969)
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by mouse MC/9 cell proliferation assay. The ED₅₀ is 0.1 ng/ml, corresponding to a specific activity of 1.0 x 10⁷ Units/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8331	Recombinant Mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF)	N/A	N/A	BA ELISA std
34-8331	Carrier-Free Recombinant Mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF)	N/A	N/A	BA ELISA
39-8331	Single-Use ELISA RSG Standard Recombinant Mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF)	N/A	N/A	ELISA

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Description

Mouse Granulocyte/Macrophage Colony Stimulating Factor (GM-CSF) is an ~14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-α, IL-2, IL-1, and IFN-γ. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse GM-CSF is biologically active and can promote proliferation of mouse MC/9 cells in culture. It is also used as a standard cytokine in mouse GM-CSF ELISA.

Applications Tested

This reagent has been tested by sandwich ELISA using the anti-mouse GM-CSF antibody pair (MP1-22E9/MP1-31G6) and in bioassays using the mouse cell line MC/9. The ED₅₀ measured in a MC/9 proliferation assay is typically 100 pg/ml, corresponding to a specific activity of approximately 1.0 x10⁷ Units/mg.

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