Product Information

Contents: Recombinant Rat Interferon gamma (IFN-γ)
Catalog Number: 14-8315
Sizes: 10 ug, 20 ug
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by mouse L929/EMCV cell protection assay. The ED₅₀ is 100 pg/ml, corresponding to a specific activity of 1.0 x 10⁷ Units/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8315	Recombinant Rat IFNg (Interferon-gamma, IFN-g)	N/A	N/A	BA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The recombinant rat IFN-γ is an ~20 kDa factor produced by activated T, B and NK cells and an anti-viral and anti-parasitic cytokine. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ is not important for its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant rat IFN-γ is biologically active and can protect L929 cell line against viral infection in culture. It is also used as standard cytokine in rat IFN-γ ELISA.

Applications Tested

This recombinant rat IFN-γ has been tested by bioassay using mouse fibroblast cell line L929. The ED₅₀ measured in a L929 protection is <100 pg/ml, corresponding to a specific activity of >1x10⁷ Units/mg.

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