Product Information

Contents: Recombinant Mouse IFNa4 (Interferon-alpha 4, IFN-a4)
Catalog Number: 14-8313
Sizes: 5 ug, 25 ug
Formulation: Sterile liquid; 20 mM Tris-HCl, 0.15 M NaCl, pH 7.5, 1.0% BSA. 0.22 μM filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids Cys25-Glu186 of mature mouse IFNa4 (accession # NM_010504).
Molecular Mass: The protein has a predicted molecular mass of 21,535 daltons. The DTT reduced and non-reduced proteins migrate as a 20 kDa polypeptide on SDS-PAGE.
Purity: Greater than 98% as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: The recombinant mouse IFN-α4 has been tested for inhibition of the cytopathic effect of EMC virus on L929 cells. The ED₅₀ for this effect is typically 40 pg/ml, corresponding to a specific activity of 2 x 10⁷ U/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8313	Recombinant Mouse IFNa4 (Interferon-alpha 4, IFN-a4)	N/A	N/A	BA ELISA std
34-8313	Carrier-Free Recombinant Mouse IFNa4 (Interferon-alpha 4, IFN-a4)	N/A	N/A	BA ELISA std

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Description

IFN-α4 is a type I interferon, previously known as B-cell interferon, leukocyte interferon, lymphoblast interferon, and pH2-stable interferon. IFN-α4 is one of at least 23 different known variants of IFN-α. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. IFN-α forms are produced by monocytes/macrophages, lymphoblastoid cells, fibroblasts, and a number of different cell types following induction by viruses, nucleic acids, glucocorticoid hormones, and low-molecular weight substances (n-butyrate, 5-bromodeoxyuridine). IFN-α4 demonstrates antiviral, antiparasitic,and antiproliferative activities.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The recombinant mouse IFNa4 has been reported useful for bioassay and ELISA.

Applications Tested

The recombinant mouse IFN-α4 has been tested for inhibition of the cytopathic effect of EMC virus on L929 cells. The ED₅₀ for this effect is typically 40 pg/ml, corresponding to a specific activity of 2 x 10⁷ U/mg.

Related Products

Cat. 14-8312 Recombinant Mouse IFNa2 (Interferon-alpha 2, IFN-a2)
Cat. 14-8316 Recombinant Human IFNa2 (Interferon-alpha 2, IFN-a2)