Product Information

Contents: Recombinant Mouse IFNa2 (Interferon-alpha 2, IFN-a2)
Catalog Number: 14-8312
Sizes: 5 ug, 25 ug
Formulation: Sterile liquid; 20 mM sodium phosphate, pH 7.2, 150 mM NaCl, with 0.5% BSA. 0.22 μM filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids Cys24-Glu190 of mature mouse IFNa2 (accession # NM_010503).
Molecular Mass: The protein has a predicted molecular mass of 19,361. The non-reduced protein migrates as a 16 kDa polypeptide on SDS-PAGE. The DTT reduced protein migrates as an 18 kDa polypeptide.
Purity: Greater than 98% as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: The recombinant mouse IFN-α2 has been tested for inhibition of the cytopathic effect of EMC virus on L929 cells. The ED₅₀ for this effect is typically 0.1 ng/ml, corresponding to a specific activity of 1 x 10⁷ U/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8312	Recombinant Mouse IFNa2 (Interferon-alpha 2, IFN-a2)	N/A	N/A	BA ELISA std
34-8312	Carrier-Free Recombinant Mouse IFNa2 (Interferon-alpha 2, IFN-a2)	N/A	N/A	BA ELISA std

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Description

IFN-α2 is a type I interferon, previously known as B-cell interferon, leukocyte interferon, lymphoblast interferon, and pH2-stable interferon. IFN-α2 is one of at least 23 different known variants of IFN-α. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. IFN-α forms are produced by monocytes/macrophages, lymphoblastoid cells, fibroblasts, and a number of different cell types following induction by viruses, nucleic acids, glucocorticoid hormones, and low-molecular weight substances (n-butyrate, 5-bromodeoxyuridine). IFN-α2 demonstrates antiviral, antiparasitic, antiproliferative activities.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The recombinant mouse IFNa2 has been reported useful for bioassay and ELISA.

Applications Tested

The recombinant mouse IFN-α2 has been tested for inhibition of the cytopathic effect of EMC virus on L929 cells. The ED₅₀ for this effect is typically 0.1 ng/ml, corresponding to a specific activity of 1 x 10⁷ U/mg.