Contents: Human IL-31 Recombinant Protein Catalog Number: 14-8310 Formulation: Sterile liquid; 0.1M Glycine, pH 3.0 with 1.0% BSA. 0.22 µm filtered. Storage Conditions: Store at less than or equal to -70°C. Handling Conditions: For best recovery, quick-spin vial prior to opening. Use in a sterile environment Source:E. coli expressed protein consisting of amino acids Ser 24-Thr 164 of mature human IL-31 (accession # NM_001014336). Molecular Mass: The DTT reduced protein migrates as a 15 kDa polypeptide on SDS-PAGE. Purity: Greater than 97%, as determined by SDS-PAGE Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Bioactivity: Measured by induction of STAT3 activation in U-87 cells. 5 ng/mL of rhIL-31 can effectively induce STAT3 activation.
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14-8310-62
5 ug
14-8310-80
25 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
Human Interleukin-31 (IL-31) is a T-cell derived cytokine. The human IL-31 cDNA encodes a 164 amino acid precursor that contains a 23 amino acid signal peptide and a 141 amino acid mature protein. Human and mouse IL-31 share 24% amino acid sequence identity in the mature region. IL-31 is mainly associated with activated T cells and preferentially expressed by Th2 rather than Th1 cells. IL-31 signals via a heterodimeric receptor complex composed of a 120 kDa, gp130-like molecule termed IL-31RA (also GPL and GLM-R) and a 180 kDa oncostatin M receptor (OSM Rb). GPL/OSMR signaling is a strong activator of STAT3 and STAT5, and can also activate STAT1, Jak1, and Jak2 signaling pathways. IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and MAP kinase pathway. The IL-31 receptor is constitutively expressed by keratinocytes and upregulated by IFN-g on monocytes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Purified Recombinant human IL-31 has been reported for use in cytokine bioassays.
Applications Tested
This recombinant human IL-31 has been tested by bioassays using the cell line U-87.
References
Chattopadhyay S, et al. Interleukin-31 and oncostatin-M mediate distinct signaling reactions and response patterns in lung epithelial cells. J Biol Chem. 2007 2;282(5):3014-26.
Neis MM, Peters B, at al. Enhanced expression levels of IL-31 correlate with IL-4 and IL-13 in atopic and allergic contact dermatitis. J Allergy Clin Immunol. 2006 118(4):930-7.
Dillon SR, et al. Interleukin 31, a cytokine produced by activated T cells, induces dermatitis in mice. Nat Immunol. 2004 5(7):752-60.
