Product Information

Contents: Recombinant mouse IL-21 (IL-21)
Catalog Number: 14-8211
Sizes: 5 ug, 25 ug
Concentration: 100 ug/ml
Formulation: Sterile liquid; 20 mM NaH2PO4, pH 7.2, 0.2 M NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids Pro25-Ser146 of mature mouse IL-21 (accession # NM_021782).
Molecular Mass: The DTT reduced protein migrates as a 15 kDa polypeptide on SDS-PAGE. The non-reduced protein migrates as a 15 kDa polypeptide.
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by dose-dependent induction of proliferation of B9 cell line. The ED50 is typically 300 pg/ml, corresponding to a specific activity of 3 x 10E6 Units/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8211	Recombinant Mouse IL-21 (Interleukin-21, IL21)	N/A	N/A	BA ELISA std
34-8211	Carrier-Free Recombinant Mouse IL-21 (Interleukin-21, IL21)	N/A	N/A	BA ELISA std

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Description

Mouse Interleukin-21 (IL-21) is a 146-amino acid protein with 57% identity to the human gene. It contains a 24-amino acid signal peptide and a 4-helix-bundle cytokine domain homologous to IL-2, IL-4 and IL-15. IL-21 stimulates B cell proliferation in an anti-CD40 dependent manner but inhibits B cell proliferation stimulated by IL-4 plus anti-IgM. IL-21 is induced by IL-6 in activated T cells, a process that is dependent on STAT3 but not on ROR-gamma. IL-21 induces Th17 differentiation and suppresses FOXP3 expression, which requires STAT3 and ROR-gamma.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-21 is biologically active and has been reported for use in ELISA and bioassay.

Applications Tested

This IL-21 protein has been tested by bioassay, yielding an ED50 of 300 pg/ml, corresponding to a specific activity of approximately 3x10E6 Units/mg.

References

Korn, T. et al. 2007. Nature. 448:484-487