Contents: Human IL-20 Recombinant Protein Catalog Number: 14-8209 Concentration: 0.1 mg/mL Formulation: Sterile liquid, phosphate buffered saline with 1% BSA, pH 7.2 Storage Conditions: Store at less than or equal to -70°C. Handling Conditions: For best recovery, quick-spin vial prior to opening. Use in sterile environment. Source:E. coli expressed amino acids Leu25-Glu-176, accession number NP_061194 Molecular Mass: 17.7 kDa Purity: Greater than 97%, as determined by SDS-PAGE Endotoxin Level: Less than 0.1 ng/µg cytokine as determined by the LAL assay. Bioactivity: The ED50 of this protein, as measured by inhibition of HK-2 cell proliferation, is 16.12 μg/ml. This corresponds to a specific activity of 62 Units/mg.
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14-8209-62
5 ug
14-8209-80
25 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
IL-20 is a recently-discovered member of the IL-10 cytokine family. It is expressed by monocytes, dendritic cells, and keratinocytes. IL-20 activates STAT3 in target cells by activating either of two heterodimeric receptor complexes. One of these consists of IL-20Rβ and IL-20Rα and can be activated by IL-19 and IL-24 as well as IL-20. The other, a complex of IL-20Rβ and IL-22Rα, is activated only by IL-20 and IL-24. IL-20 induces the production of proinflammatory cytokines such as IL-1β, IL-6, and TNFα in monocytes and has been implicated in inflammatory conditions such as atherosclerosis, rheumatoid arthritis, and psoriasis. IL-20 also induces the proliferation of hematopoetic progenitor cells and keratinocytes
Applications Reported
For research use only, not for diagnostic or therapeutic use. Human IL-20 Recombinant Protein is biologically active.
Applications Tested
The ED50 of this protein, as measured by inhibition of HK-2 cell proliferation, is 16.12 μg/ml. This corresponds to a specific activity of 62 Units/mg.
References
Wolk K, Witte K, Witte E, Proesch S, Schulze-Tanzil G, Nasilowska K, Thilo J, Asadullah K, Sterry W, Volk HD, Sabat R. Maturing dendritic cells are an important source of IL-29 and IL-20 that may cooperatively increase the innate immunity of keratinocytes. J Leukoc Biol. 2008 May;83(5): 1181-93.
Chen WY, Cheng BC, Jiang MJ, Hsieh MY, Chang MS. IL-20 is expressed in atheroscleroris plaques and promotes atherosclerosis in apolipoprotein E-deficient mice. Arterioscler Thromb Vasc Biol. 2006 Sep;26(9):2090-5.
Hsu YH, Li HH, Hsieh MY, Liu MF, Huang KY, Chin LS, Chen PC, Cheng HH, Chang MS. Function of interleukin-20 as a proinflammatory molecule in rheumatoid and experimental arthritis. Arthritis Rheum. 2006 Sep;54(9):2722-33.
