Product Information

Contents: Recombinant Mouse IL-17A (Interleukin-17A)
Catalog Number: 14-8171
Sizes: 2 ug, 5 ug, 25 ug
Formulation: Sterile liquid; 20 mM NaHPO4, 0.2M NaCl with 1.0% BSA. 0.22 uM filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids thr 22-ala 158 of mature mouse IL-17A (accession # NM_010552).
Molecular Mass: The protein does not contain an N-terminal methionine. The polypeptide has a predicted molecular mass of 15,377. The DTT reduced protein migrates as a 15 kDa polypeptide. The nonreduced cystine linked protein migrates as a 30 kDa protein on non-reducing SDS-PAGE.
Purity: Greater than 98% as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by induction of IL-6 production by NIH/3T3 cells. The ED₅₀ is 3.0 ng/ml, corresponding to a specific activity of 3.3 x 10⁵ Units/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8171	Recombinant Mouse IL-17A (Interleukin-17A, IL17A)	N/A	N/A	BA ELISA std
34-8171	Carrier-Free Recombinant Mouse IL-17A (Interleukin-17A, IL17A)	N/A	N/A	BA ELISA std
39-8171	Single-Use ELISA RSG Standard Recombinant Mouse IL-17A (Interleukin-17A, IL17A)	N/A	N/A	ELISA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A binds to a receptor that binds also to HVS13 (viral IL-17A) and to CTLA-8.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-17A is biologically active and can promote IL-6 production in vitro. Recombinant mouse IL-17A is useful as an ELISA standard.

Applications Tested

The recombinant mouse IL-17A has been tested as the standard in a mouse IL-17 sandwich ELISA and in bioassay for induction of IL-6 production by NIH/3T3 cells. The ED50 is 3.0 ng/ml, corresponding to a specific activity of 3.3 x 10E5 Units/mg.

References

Fossiez, F., et al. 1996. T cell interleukin-17 induces stromal cells to produce proinflammatory and hematopoietic cytokines. J. Exp. Med. 183: 2593-2603.
Kennedy, J., et al. 1996. Mouse IL-17: A cytokine preferentially expressed by alpha beta TCR+CD4 -CD8 - T cells. J. Interferon Cytokine Res. 16: 611-617.
Hurst, S., et al. 2002. New IL-17 family members promote Th1 or Th2 responses in the lung: in vivo function of the novel cytokine IL-25. J. Immunol. 169: 443-453.

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