Product Information

Contents: Recombinant Mouse Interleukin-15 (IL-15)
Catalog Number: 14-8151
Sizes: 2 ug, 5 ug
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.1 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by mouse CTLL2 cell proliferation assay. The ED₅₀ is 100.0 ng/ml, corresponding to a specific activity of 10⁴ Units/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8151	Recombinant Mouse IL-15 (Interleukin-15, IL15)	N/A	N/A	BA
34-8151	Carrier-Free Recombinant Mouse IL-15 (Interleukin-15, IL15)	N/A	N/A	BA ELISA std
39-8151	Single-Use ELISA RSG Standard Recombinant Mouse IL-15 (Interleukin-15, IL15)	N/A	N/A	ELISA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Mouse IL-15 is a 14-15 kDa factor, previously known as IL-T. IL-15 is produced by a variety of cells and has a role in the T cell-mediated immune response and the central nervous system. IL-15 binds to the β and γ subunits of the IL-2 receptor; however, these two cytokines have distinct biological activities. IL-15 is a specific maturation factor for NK cells and mimics the BM microenvironment for NK cell development in vitro. It is reported that IL-15 delivers an anti-apoptotic and survival signal for NK, T, and B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-15 is biologically active.

Applications Tested

This recombinant mouse IL-15 has been tested in CTLL-2 cell proliferation assay with an observed ED₅₀ of 100.0 ng/ml, corresponding to a specific activity of approximately 10⁴ Units/mg.

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