Product Information

Contents: Recombinant Human Interleukin-13 (IL-13)
Catalog Number: 14-8139
Sizes: 2 ug, 5 ug, 25 ug
Formulation: Sterile liquid; 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, with 0.5% BSA. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids Gly 21-Leu 132 of mature human IL-13 (accession #NM_002188).
Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 10,591. The DTT reduced protein migrates as a 9 kDa polypeptide on SDS-PAGE and the non-reduced protein migrates with slightly greater mobility.
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by human TF-1 cell proliferation assay. The ED₅₀ is typically 1.0-5.0 ng/ml, corresponding to a specific activity of 0.2-1.0 x 10⁶ Units/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8139	Recombinant Human IL-13 (Interleukin-13, IL13)	N/A	N/A	BA ELISA
34-8139	Carrier-Free Recombinant Human IL-13 (Interleukin-13, IL13)	N/A	N/A	BA ELISA
39-8139	Single-Use ELISA RSG Standard Recombinant Human IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Human interleukin-13 (IL-13), also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8⁺ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression.

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Applications Tested

This recombinant human IL-13 has been tested by a sandwich ELISA using the anti-human IL-13 ELISA antibody pair (PVM13-1 / polyclonal anti-human IL-13), and in bioassays using the IL-13 responsive cell line TF-1. The ED₅₀ as determined by the dose-dependent proliferation of TF-1 cells is typically 1.0-5.0 ng/ml, corresponding to a specific activity of 0.2-1.0 x 10⁶ Units/mg.

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