Contents: Human IL-10 Recombinant Protein Catalog Number: 14-8109 Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 0.5% BSA. 0.22 µm filtered. Storage Conditions: Store at less than or equal to -70°C. Handling Conditions: For best recovery, quick-spin vial prior to opening. Use in a sterile environment Source: E. coli expressed amino acids Ser 19-Asn 178 of mature human IL-10 (accession # NM_000572). Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 18,647. The DTT reduced protein migrates as an 18 kDa polypeptide on SDS-PAGE and the non-reduced protein migrates with slightly greater mobility. Purity: Greater than 98%, as determined by SDS-PAGE Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Bioactivity: Measured by mouse MC/9 cell proliferation assay, costimulated with IL-4. The ED50 is 1.0 ng/ml, corresponding to a specific activity of 1.0 x 106 Units/mg.
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14-8109-62
5 ug
14-8109-80
25 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
Human IL-10 is a ~18 kDa factor also known as cytokine synthesis inhibitory factor (CSIF). Th2 T cells, B1 B cells and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells and acts as a costimulatory signal for mast cells, developing thymocytes and Th2 response.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant human IL-10 is biologically active and can promote proliferation of MC/9 cell line in culture. It is also used as standard cytokine in human IL-10 ELISA.
Applications Tested
This recombinant human IL-10 has been tested by sandwich ELISA using anti-human IL-10 antibody pair (JES3-9D7/JES3-12G8) and in bioassay using mouse cell line MC/9. The ED50 measured in a MC/9 proliferation assay is typically 1.0 ng/ml, corresponding to a specific activity of approximately 1x106 Units/mg.
