Product Information

Contents: Recombinant Human Interleukin-8 (IL-8)
Catalog Number: 14-8089
Sizes: 10 ug, 25 ug
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids Ser 29-Ser 99 of human IL-8 (accession # NM_000584).
Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 8,386. The DTT reduced protein migrates as an 8 kDa polypeptide and the non-reduced polypeptide migrates as a 9kDa polypeptide on SDS-PAGE.
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by chemotaxis assay of human peripheral blood neutrophils. Maximal chemoattractant activity was observed between 10-100 ng/ml.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8089	Recombinant Human IL-8 (Interleukin-8, IL8) 72aa	N/A	N/A	BA
34-8089	Carrier-Free Recombinant Human IL-8 (Interleukin-8, IL8) 72aa	N/A	N/A	BA

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Description

Human IL-8, also called neutrophil attractant/activating protein (NAP-1), monocyte derived neutrophil activating peptide (MONAP), monocyte derived neutrophil activating factor (ANF), monocyte derived neutrophil chemotactic factor (MDNCF), and neutophil activating factor (NAF), is a ~6-8 kDa factor produced by variety types of cells including monocytes, lymphocytes, granulocytes, fibroblasts and endothelial cells. IL-8 is an inflammatory cytokine which functions as a neutrophil chemoatractant and activating factor. It also attracts basophils and a subpopulation of lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant human IL-8 is biologically active.

Applications Tested

This recombinant human IL-8 has been tested in bioassays for chemoattractant activity, with maximal activity between 10-100 ng/ml.