Product Information

Contents: Recombinant Human Interleukin-5 (IL-5)
Catalog Number: 14-8059
Sizes: 2 ug, 5 ug
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by human TF-1 cell proliferation assay. The ED₅₀ is 0.1 ng/ml, corresponding to a specific activity of 1.0 x 10⁷ Units/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8059	Recombinant Human IL-5 (Interleukin-5, IL5)	N/A	N/A	BA ELISA
39-8059	Single-Use ELISA RSG Standard Recombinant Human IL-5 (Interleukin-5, IL5)	N/A	N/A	ELISA

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Description

Human IL-5 (also known as BCGF and BCDF) is a ~40 kDa factor produced mainly by T cells. IL-5 is present as an N-glycosylated homodimer and is a hematopoietic growth factor promoting proliferation, activation and differentiation of eosinophil granulocytes. In the human, IL-5 also supports generation of cytotoxic T cells. In the mouse, IL-5 acts on B cells to induce proliferation and secretion of IgM and IgA from endotoxin activated B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant human IL-5 is biologically active and can promote proliferation of human TF-1 cell line in culture. It is also used as standard cytokine in human IL-5 ELISA.

Applications Tested

This recombinant human IL-5 has been tested by sandwich ELISA using anti-human IL-5 antibody pair (TRFK5/JES1-5A10) and in bioassays using human TF-1 cell line. The ED₅₀ measured in a TF-1 proliferation assay is typically 100 pg/ml, corresponding to a specific activity of approximately 1x10⁷ Units/mg.

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