Product Information

Contents: Recombinant Rat Interleukin-1β (IL-1β)
Catalog Number: 14-8010
Sizes: 5 ug
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by mouse D10S cell proliferation assay. The ED₅₀ is 0.1 ng/ml, corresponding to a specific activity of 1.0 x 10⁷ U/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8010	Recombinant Rat IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	BA

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Description

The rat IL-1β, also called lymphocyte activating factor (LAF), endogenous pyrogen (EP), leucocyte endogenous mediator (LEM), mononuclear cell factor (MCF), is an ~17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1β is mostly cell associated with 23% amino acid homology with IL-1α. The immune regulatory role of IL-1β is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant rat IL-1β is biologically active and has been reported for use in cytokine bioassays. [For a rat IL-1β ELISA standard, please consult eBioscience Technical Support. Product 14-8010 is a bioactive protein, but is not recommended for rat IL-1β sandwich ELISA.]

Applications Tested

This reagent has been tested in bioassays with mouse D10S cells. The ED₅₀ is less than 0.1 ng/ml, corresponding to a specific activity of greater than 1x10⁷ Units/ml. [For a rat IL-1β ELISA standard, please consult eBioscience Technical Support. Product 14-8010 is a bioactive protein, but is not recommended for rat IL-1β sandwich ELISA.]

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