Contents: Mouse PDGF-BB Recombinant Protein Catalog Number: 14-8003 Concentration: 0.1 mg/mL Formulation: Sterile liquid, phosphate buffered saline with 1% BSA, pH 7.2 Storage Conditions: Store at less than or equal to -70°C. Handling Conditions: For best recovery, quick-spin vial prior to opening. Use in sterile envrioment. Source:E. coli expressed Molecular Mass: 24.6 kDa Purity: Greater than 97%, as determined by SDS-PAGE Endotoxin Level: Less than 0.1 ng/µg cytokine as determined by the LAL assay. Bioactivity: The ED50 of this protein, as measured by mouse 3T3 fibroblast proliferation assay, is 1.72 ng/ml. This corresponds to a specific activity of 5.8 x 105 Units/mg.
Product Options
Cat. No.
Size
Price
Add Qty to Cart
14-8003-62
5 ug
14-8003-80
25 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
PDGF-BB is a member of the platelet-derived growth factor family, a group of proteins that is released by platelets upon activation. The PDGF proteins are dimers of two of four peptide chains designated A, B, C, and D. PDGF-BB is formed by two B-chains, while PDGF-AB is one B chain and one A-chain forming the only heterodimer in the family. PDGF-BB is unique among the family in that it can bind to and activate all three forms of the PDGF receptor. It induces the proliferation, migration, and survival of fibroblasts, endothelial cells, epithelial cells, and smooth muscle cells. PDGF-BB plays an important role in angiogenesis and smooth muscle development during embryogenesis, and is important for wound healing and regeneration in adults. High levels of PDGF-BB are often detected in malignant tumors, and it is believed to facilitate tumor metastasis and angiogenesis.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Mouse PDGF-BB Recombinant Protein is biologically active.
Applications Tested
The ED50 of this protein, as measured by mouse 3T3 fibroblast proliferation assay, is 1.72 ng/ml. This corresponds to a specific activity of 5.8 x 105 Units/mg.
References
Nissen LJ, Cao R, Hedlund EM, Wang Z, Zhao X, Wetterskog D, Funa K, Brakenhielm E, Cao Y. Angiogenic factors FGF2 and PDGF-BB synergistically promote murine tumor neovascularization and metastasis. J Clin Invest. 2007 Oct;117(10):2766-77.
Cao Y. Direct role of PDGF-BB in lymphangiogenesis and lymphatic metastasis. Cell Cycle. 2005 Feb;4(2):228-30.
Kamimura M, Bea F, Akizawa T, Katus HA, Kreuzer J, Viedt C. Platelet-derived growth factor induces tissue factor expression in vascular smooth muscle cells via activation of Egr-1. Hypertension. 2004 Dec;44(6):944-51.
