Contents: Polyclonal rabbit anti-mouse XCL1 (lymphotactin/SCM-1α) (neutralizing) Catalog Number: 14-7997 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Clone: Polyclonal Host/Isotype: Rabbit IgG Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. The 50µg size of this antibody is supplied in liquid form. The 200µg size of this antibody may be supplied in liquid or lyophilized powder forms. To reconstitute the lyophilized antibody, add 200µl of PBS for a final concentration of 1mg/ml.
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14-7997-81
50 ug
14-7997-83
200 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The polyclonal antibody reacts with mouse lymphotactin, also known as XCL1, and was generated using E. coli-expressed amino acid 1-92 of mature mouse lymphotactin as an immunogen. XCL1 is the only C chemokine so far identified which has a single cysteine residue near the amino terminus. It has 114 amino acids with a 22-amino acid signal peptide. It is produced in activated CD8+ T cells and NK cells. XCL1 attracts both T lymphocytes and NK cells. Cross-reactivity to XCL1 of other species has not been determined.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoprecipitation, and immunoblotting (WB).
Applications Tested
The tested titer for Western blot is 1:2000; for immunoprecipitation, use 1:500. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Kelner, G.S. et al. 1994. Lymphotactin: a cytokine that represents a new class of chemokine. Science 266:1395-1399 Hedrick, J.A. et al. 1997. Lymphotactin is produced by NK cells and attracts both NK cells and T cells in vivo. J Immunol 158:1533-1540
