Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The polyclonal antibody reacts with both mouse and rat RANTES and was generated using E. coli-expressed amino acid 1-70 of mature rat RANTES as an immunogen. RANTES (regulated upon activation, normal T-cell expressed and secreted) is a 70-amino acid CC chemokine originally cloned from T cell-specific sequence-enriched cDNA libraries. Both human and murine RANTES are chemotactic for monocytes. Human RANTES is also a chemoattractant for T cells and eosinophils, as well as an activator of basophils for histamine release. In contrast to other CC chemokines, RANTES is only slowly expressed after activation of leukocytes. It is involved in a number of pathological conditions like chronic inflammation and autoimmune diseases.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB). Cross-reactivity to CCL5 of other species has not been determined.
Applications Tested
The tested titer for Western blot is 1:1000. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Schall, T.J. 1988. A human T cell-specific molecule is a member of a new gene family. J Immunol. 141:1018-1025 Schall, T. et al. 1992. Molecular cloning and expression of the murine RANTES cytokine: structural and functional conservation between mouse and man. Eur J Immunol 22:1477 Neilson,E.G., et al. 1992. Isolation and characterization of cDNA from renal tubular epithelium encoding murine Rantes: A small intercrine from the Scy superfamily. Kidney Int. 41:220-225 Haelens, A. et al., 1996. Leukocyte migration and activation by murine chemokines. Immunobiol. 195:499-521
