Contents: Anti-Mouse/Rat CXCL12 alpha (SDF-1 alpha) Purified Catalog Number: 14-7992 Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary. The 50µg size of this antibody is supplied in liquid form. The 200µg size of this antibody may be supplied in liquid or lyophilized powder forms. Storage Conditions: Store at 2-8°C. Clone: Polyclonal Host/Isotype: Rabbit IgG
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Size
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14-7992-81
50 ug
14-7992-83
200 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The polyclonal antibody reacts with both mouse and rat SDF-1α and was generated using E. coli-expressed mouse SDF-1α as an immunogen. SDF-1α (stromal cell-derived factor-1 alpha), also known as pre-B-cell growth-stimulating factor (PBSF), is a 70-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of the SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis. SDF-1 has been shown to be chemotactic for lymphocytes. In addition, SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV-1 entry.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal has been reported for use in immunoblotting (WB), immunoprecipitation, and immunohistochemistry. Cross-reactivity to SDF-1α of other species has not been determined.
Applications Tested
To achieve best results, titrate antibody by dilution for each application as follows: Western Blot 1:1000 Immunoprecipitation 1:300-500 Immunohistochemistry 1:100-500
References
Tashiro, K. et al. 1993. Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins. Science 261:600-603. Nagasawa, T et al. 1994. Molecular cloning and structure of a pre-B-cell growth-stimulating factor. Proc. Natl. Acad. Sci. USA 91:2305-2309. Nagasawa, T. et al. 1996. Defeats of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1. Nature 328:635-638. Bleul. C.C. et al. 1996. A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1). J. EXP. Med. 184:1101-1109. Bleul. C.C. et al. 1996. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature 382:829-833. Oberlin E. et al. 1996. The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by C-cell-line-adapted HIV-1. Nature 382:833-835. Jung Y, Ryu K, et al. 2006. Syngenic bone marrow cells restore hepatic function in carbon tetrachloride-induced mouse liver injury. Stem Cells Dev. 15(5):687-95. (IHC paraffin, PubMed) Otsuru S, Tamai K, et al. 2008. Circulating bone marrow-derived osteoblast progenitor cells are recruited to the bone-forming site by the CXCR4/stromal cell-derived factor-1 pathway. Stem Cells. 26(1):223-34. (IHC frozen, PubMed)
