The 50µg size of this antibody is supplied in liquid form. The 200µg size of this antibody may be supplied in liquid or lyophilized powder forms. To reconstitute the lyophilized antibody, add 200µl of PBS for a final concentration of 1mg/ml.
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Cat. No.
Size
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14-7991-81
50 ug
14-7991-83
200 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The polyclonal antibody reacts with both mouse and rat CXCL12 β (SDF-1β) and was generated using E. coli-expressed mouse CXCL12 β as an immunogen. CXCL12 β, also known as pre-B-cell growth-stimulating factor (PBSF) and stromal cell-derived factor-beta (SDF-1β), is a 74-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of CXCL12 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis in mice. CXCL12 has been shown to be chemotactic for lymphocytes. In addition, CXCL12 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. CXCL12 binding to CXCR4 inhibits HIV-1 entry.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal can be used in immunoblotting (WB), immunoprecipitation, and immunohistochemistry. Cross-reactivity to CXCL12 β of other species has not been determined.
Applications Tested
To achieve best results, titrate antibody by dilution for each application as follows: Western Blot 1:1000 Immunoprecipitation 1:300-500 Immunohistochemistry 1:100-500.
References
Tashiro, K. et al. 1993. Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins. Science 261:600-603. Nagasawa, T et al. 1994. Molecular cloning and structure of a pre-B-cell growth-stimulating factor. Proc. Natl. Acad. Sci. USA 91:2305-2309. Nagasawa, T. et al. 1996. Defeats of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1. Nature 328:635-638. Bleul. C.C. et al. 1996. A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1). J. EXP. Med. 184:1101-1109. Bleul. C.C. et al. 1996. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature 382:829-833. Oberlin E. et al. 1996. The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by C-cell-line-adapted HIV-1. Nature 382:833-835.
