Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
The rabbit polyclonal antibody reacts with recombinant and natural human and rat CX3CR1; the antibody was generated using E. coli-expressed human CX3CR1 amino terminal domain as an immunogen. CX3CR1, also termed neurotactini or fractalkine, is a membrane-bound CX3C chemokine. Human CX3CR1, also named V28, was first cloned from human genomic DNA libraries. A G-protein-coupled seven-transmembrane domain receptor, it was recently identified to serve as fractalkine receptor. Human CX3CR1 has been shown to mediate both the adhesive and migratory functions of fractalkine. Fractalkine and CX3CR1 represent new types of leukocyte trafficking regulators. Cross reactivity to CX3CR1 of other species has not been determined.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections.
Applications Tested
To achieve best results, titrate antibody by dilution for each application:
Western Blot 1:2000-1:5000 Immunoprecipitation 1:300-1:800 Immunohistochemistry 1:100-1:500
References
Bazan J.F. et al. 1997. A new class of membrane-bound chemokine with a CX3C motif. Nature 385:640-644
Harrison, J.K. et al. 1994. cDNA cloning of a G-protein-coupled receptor expressed in rat spinal cord and brain related to chemokine receptors. Neurosci Lett 169:85-89
Imai, T. et al. 1997. Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell 91:521-530
Combadiere C. et al. 1998. Identification of CX3CR1. A chemotactic receptor for the human CX3C chemokine fractalkine and a fusion coreceptor for HIV. J Biol Chem 273(37):23799-23804
Hung S, Pochampally RR, et al. 2007. Short-term exposure of multipotent stromal cells to low oxygen increases their expression of CX3CR1 and CXCR4 and their engraftment in vivo. PLoS ONE. 2(5):e416. (WB, PubMed)
