mouse/human Foxp3 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-mouse/human Foxp3
Catalog Number: 14-7979
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio7979
Isotype: Mouse IgG1

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14-7979-80

25 ug

14-7979-82

100 ug

Immunoblotting of PBMC (Lane A), BALB/c splenocyte (Lane B), or recombinant human Foxp3 fusion protein (Lane C). Lysates were directly sonicated in Laemmli sample buffer and 10⁶ cells/lane were loaded. Blots were probed with 1 ug/ml eBio7979 and revealed with HRP anti-mouse IgG. Foxp3 is detected at ~50 kDa with a reported splice variant in human tissue slightly lower.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7979	Affinity Purified anti-mouse/human Foxp3	N/A	N/A	IH/F IHC(Paraffin) IP WB
12-7979	Phycoerythrin (PE) anti-mouse/human Foxp3	488	575	IC Flow
51-7979	Alexa Fluor® 647 anti-mouse/human Foxp3	633	668	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

eBioscience offers a panel of monoclonal antibodies to different epitopes of human and mouse Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells. Other antibodies to human Foxp3 include PCH101 (cat. 72-5776) and 236A/E7 (cat. 12-4777). Additional antibodies to mouse Foxp3 include FJK-16s (cat. 72-5775) and NRRF-30 (cat. 12-4771).

The eBio7979 antibody reacts with human and mouse foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Please see the following link for FAQ regarding the usage of eBioscience Foxp3 reagents: http://www.ebioscience.com/ebioscience/Foxp3FAQs.htm

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio7979 antibody has been reported for use in immunoblotting (WB).

Applications Tested

This eBio7979 antibody has been tested by immunoblotting of normal human peripheral blood mononuclear cell lysates at 1-2 μg/ml.

References

Sakaguchi S. 2003. The origin of FOXP3-expressing CD4+ regulatory T cells: thymus or periphery. J Clin Invest. 112(9):1310-2.

Hori S, Nomura T, Sakaguchi S. 2003. Control of regulatory T cell development by the transcription factor Foxp3. Science. 299(5609):1057-61.

Takahata Y, Nomura A, Takada H, Ohga S, Furuno K, Hikino S, Nakayama H, Sakaguchi S, Hara T. 2004. CD25+CD4+ T cells in human cord blood: an immunoregulatory subset with naive phenotype and specific expression of forkhead box p3 (Foxp3) gene. Exp Hematol. 32(7):622-9.

Xystrakis E, Dejean AS, Bernard I, Druet P, Liblau R, Gonzalez-Dunia D, Saoudi A. 2004. Identification of a novel natural regulatory CD8 T-cell subset and analysis of its mechanism of regulation. Blood. 104(10):3294-301.

Manavalan JS, Kim-Schulze S, Scotto L, Naiyer AJ, Vlad G, Colombo PC, Marboe C, Mancini D, Cortesini R, Suciu-Foca N.2004. Alloantigen specific CD8+CD28- FOXP3+ T suppressor cells induce ILT3+ ILT4+ tolerogenic endothelial cells, inhibiting alloreactivity. Int Immunol. 16(8):1055-68.

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