Product Information

Contents: Affinity Purified anti-mouse SLP-76 (SLP76)
Catalog Number: 14-7761
Sizes: 25 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MS76
Isotype: Mouse IgG2a

C57BL/6 splenocytes were surface-stained with APC anti-mouse CD3ε, clone 145-2C11 (cat. 17-0031). Subsequently, cells were fixed and permeabilized, stained with 0.5 μg of FITC anti-mouse SLP-76 (clone MS76) (right) or mouse IgG2a isotype control (cat. 11-4724) (left) and analyzed by flow cytometry.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7761	Affinity Purified anti-mouse SLP-76 (SLP76)	N/A	N/A	FC IP WB
11-7761	Fluorescein isothiocyanate (FITC) anti-mouse SLP-76 (SLP76)	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MS76 antibody reacts with mouse SLP-76. SLP-76 (SH2 domain-containing leukocyte protein of 76 kD) has also been described as lymphocyte cytosolic protein 2 (LCP2 ) and 76-kDa phosphoprotein (pp76 ). SLP-76 is a cytosolic adaptor protein expressed in several cell types of hematopoietic origin. SLP-76 associates with a number of additional molecules and is required for coupling receptor ligation with more distal signaling events in immature thymocytes, mature T cells, platelets, and mast cells. SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MS76 antibody has been reported to be useful for IP, Western, and intracellular staining for flow cytometric analysis.

Applications Tested

The affinity purified format of this antibody has been tested by IP (1-5 ug) and Western blotting (1 ug/ml). The fluorochrome labelled MS76 antibody has been tested by intracellular staining with flow cytometric analysis of fixed and permeabilized mouse splenocytes.

References

Cao MY, Davidson D, Yu J, Latour S, Veillette A. 1999. Clnk, a novel SLP-76-related adaptor molecule expressed in cytokine-stimulated hemopoietic cells. J Exp Med 190:1527-1534.
Clements JL, Yang B, Ross-Barta SE, Eliason SL, Hrstka RF, Williamson RA, Koretzky GA. 1998. Requirement for the leukocyte-specific adapter protein SLP-76 for normal T cell development. Science 281:416-419.
Clements JL, Lee JR, Gross B, Yang B, Olson JD, Sandra A, Watson SP, Lentz SR, Koretzky GA. 1999. Fetal hemorrhage and platelet dysfunction in SLP-76-deficient mice. J Clin Invest 103:19-25.
Boerth, NJ, Sadler, JJ, Bauer, DD, Clements, JL, Gheith, SM, and GA Koretzky. Recruitment of SLP-76 to the membrane and glycolipid-enriched membrane microdomains replaces the requirement for linker for activation of T cells in T cell receptor signaling. J. Exp. Med. 192(7):1047, 2000.

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