Affinity Purified anti-human IL-17F (Interleukin-17F)

Contents: Affinity Purified anti-human IL-17F (Interleukin-17F)
Catalog Number: 14-7479
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: H17F10A7
Isotype: Mouse IgG1

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-7479-80	25 ug	$140
14-7479-82	100 ug	$275

*International customers: Please contact your distributor for region specific pricing.

IL-17 producing cells were generated by culturing human PBMC with anti-CD3 and anti-CD28 in the presence of recombinant human IL-23 for 3 days, followed by 3 days with IL-23 alone. Day 6 cultures were treated with Brefeldin A alone (1) or PMA and Ionomycin in the presence of Brefeldin A (2) for 16 hours. Cell lysates were run under reducing conditions, immunoblotted with 2ug/ml of H17F10A7 purified anti-human IL-17F antibody and revealed with anti-mouse HRP-conjugated polyclonal antibody.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7479	Affinity Purified anti-human IL-17F (Interleukin-17F)	N/A	N/A	WB

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Description

The monoclonal antibody 17F10A7 reacts with human IL-17F, a member of the IL-17 family of pro-inflammatory cytokines. Like IL-17A, IL-17F is a disulfide-linked, homodimeric glycoprotein. The IL-17F homodimer includes a classical cysteine knot motif, which is found also in the TGF-ß, BMP, and NGF superfamilies. The presence of the cysteine knot motif suggested the possibility of a heterodimeric structure, as was reported for TGF-ß and inhibin/activin. Recent reports confirm that co-expression of IL-17F and IL-17A in HEK293 cells results in the formation of biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Moreover, activated human CD4+ T cells were found to produce the IL-17A/F heterodimer, along with the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-a secretion; IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). These heterodimers can be detected by immunoprecipitation with eBio64CAP17 anti-IL-17A monoclonal antibody followed by immunoblot with H17F10A7.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This H17F10A7 antibody has been reported for use in immunoblotting (WB).

Applications Tested

This H17F10A7 antibody has been tested at 1-2ug/ml by western blot analysis of cultured, activated Th17 cells, where it detects a band of approximately 18kDa.

It is recommended that H17F10A7 be used under reducing conditions only.

References

Wright, J.F., et al. 2007. Identification of an IL-17F/17A heterodimer in activated human CD4+ T cells. JBC. 282: 13447-13455.

Chang, S.H., et al. 2007. A novel heterodimeric cytokine consisting of IL-17 and IL-17F regulates inflammatory responses. Cell Res. Advance online publication. 24 April 2007. doi: 10.1038.

Chen Z, Tato CM, Muul L, Laurence A, O'shea JJ. Distinct regulation of interleukin-17 in human T helper lymphocytes. Arthritis Rheum. 2007 Sep;56(9):2936-46.

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