Product Information

Contents: Affinity Purified anti-human CD45RB
Catalog Number: 14-7457
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MEM-55 (MEM55)
Isotype: Mouse IgG1
HLDA No.: III

Staining of normal human peripheral blood cells with 0.5 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.5 μg of Purified anti-human CD45RB (MEM55) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and SAv-FITC (cat. 11-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7457	Affinity Purified anti-human CD45RB	N/A	N/A	FC
11-7457	Fluorescein isothiocyanate (FITC) anti-human CD45RB	488	518	FC
12-7457	Phycoerythrin (PE) anti-human CD45RB	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MEM-55 monoclonal antibody reacts with human CD45RB, the 190, 205, and 220 kDa isoforms of the leucocyte common antigen (LCA). CD45RB is expressed on a subset of B cells, T cells, monocytes, and at lower density on granulocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MEM-55 (MEM55) antibody has been reported for use in flow cytometric analysis.

Applications Tested

The MEM-55 (MEM55) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

McMichael, A.J., P.C.L. Beverly, et al. eds. 1987. Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control