Print Page

Adobe PDF

Human Cellular Antigens Chart

Contents: Affinity Purified anti-human CD45RB Catalog Number: 14-7457 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: MEM-55 (MEM55) Isotype: Mouse IgG1 HLDA No.: III Prices for This Product* Cat. No. Size Price Qty Action 14-7457-80 25 ug $60 14-7457-82 100 ug $110 *International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with 0.5 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.5 μg of Purified anti-human CD45RB (MEM55) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and SAv-FITC (cat. 11-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7457	Affinity Purified anti-human CD45RB	N/A	N/A	FC
11-7457	Fluorescein isothiocyanate (FITC) anti-human CD45RB	488	518	FC
12-7457	Phycoerythrin (PE) anti-human CD45RB	488	575	FC
15-7457	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD45RB	488	670	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

---

Description

---

The MEM-55 monoclonal antibody reacts with human CD45RB, the 190, 205, and 220 kDa isoforms of the leucocyte common antigen (LCA). CD45RB is expressed on a subset of B cells, T cells, monocytes, and at lower density on granulocytes.

---

Applications Reported

---

For research use only, not for diagnostic or therapeutic use. The MEM-55 (MEM55) antibody has been reported for use in flow cytometric analysis.

---

Applications Tested

---

The MEM-55 (MEM55) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

---

References

---

McMichael, A.J., P.C.L. Beverly, et al. eds. 1987. Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.

---

Related Products

---

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control