human TNFa antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-human TNF-α
Catalog Number: 14-7348
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MAb1
Isotype: Mouse IgG1, κ

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14-7348-81

50 ug

14-7348-85

500 ug

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7348	Affinity Purified anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	ELISA cap ELISPOT cap
16-7348	Functional Grade* Purified anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	ELISA cap ELISPOT cap NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The MAb1 antibody reacts with human tumor necrosis factor-alpha (TNF-α), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4⁺ T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane bound form.

Applications Reported

For research use only, not for diagnostic or therapeutic use. MAb1 has been reported for use in capture of human TNF-α by ELISA. It can also be used for immunoblotting (WB) and for neutralizing TNF-α binding and biological activity (use Functional Grade purified cat.16-7348).

Applications Tested

The MAb1 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human TNF-alpha (TNF-a) in combination with the biotin MAb11 (13-7349) antibody for detection and recombinant human TNFa (14-8329) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

References

Danis, V. A., G. M. Franic, et al. (1991). "Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and IL-6 on the production of immunoreactive IL-1 and TNF-alpha by human monocytes." Clin Exp Immunol 85(1): 143-50.
Rathjen, D. A., K. Cowan, et al. (1991). "Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors." Mol Immunol 28(1-2): 79-86.

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