Product Information

Contents: Affinity Purified anti-mouse TNF-alpha (TNF-α)
Catalog Number: 14-7321
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MP6-XT22
Isotype: Rat IgG1, κ

Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. The cells were surface stained with FITC anti-mouse CD4 (GK1.5) and intracellularly stained with PE anti-mouse TNF-a (MP6-Xt22). (Right) The cells were surface stained with PE anti-mouse CD4 and intracellularly stained with APC anti-mouse TNF-a.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7321	FITC anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	518	IC Flow
12-7321	PE anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	575	IC Flow
14-7321	Affinity Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	IC Flow IH/F
16-7321	Functional Grade* Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	NU
17-7321	APC anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	660	IC Flow
19-7321	Cy5 anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	670	IC Flow
51-7321	Alexa Fluor® 647 anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	668	IC Flow
53-7321	Alexa Fluor® 488 anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	519	IC Flow
57-7321	Pacific Blue® anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF-α), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4⁺ T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MP6-XT22 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunohistology staining of frozen tissue sections. (Fluorochrome conjugated MP6-XT22 is recommended for use in intracellular flow cytometry.)

Applications Tested

The MP6-XT22 antibody has been tested to block staining with fluorochrome conjugated MP6-XT22. This can be used at less than or equal to 2 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hunter CA, Litton MJ, et al. 1994. Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis. J Infect Dis. 170(4): 939-45.

Litton MJ, Sander B, et al. 1994. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J Immunol Methods 175(1): 47-58.

Abrams JS, Roncarolo MG, et al. 1992. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 127: 5-24.

Chackerian B, Lowy DR and Schiller JT. 2001. Conjugation of a self-antigen to papillomavirus-like particles allows for efficient induction of protective autoantibodies. J Clin Invest. 108(3):415-23. (IHC frozen, PubMed)

Williams RO, Mauri C, et al. 1998. Therapeutic actions of cyclosporine and anti-tumor necrosis factor alpha in collagen-induced arthritis and the effect of combination therapy. Arthritis Rheum. 41(10):1806-12. (IHC frozen, PubMed)

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