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Contents: Affinity Purified anti-mouse TNF-alpha (TNF-α) Catalog Number: 14-7321 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: MP6-XT22 Isotype: Rat IgG1, κ Prices for This Product* Cat. No. Size Price Qty Action 14-7321-81 50 ug $80 14-7321-85 500 ug $220 *International customers: Please contact your distributor for region specific pricing.

Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. The cells were surface stained with FITC anti-mouse CD4 (GK1.5) and intracellularly stained with PE anti-mouse TNF-a (MP6-Xt22). (Right) The cells were surface stained with PE anti-mouse CD4 and intracellularly stained with APC anti-mouse TNF-a.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7321	FITC anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	518	IC Flow
12-7321	PE anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	575	IC Flow
14-7321	Affinity Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	IC Flow  IH/F
16-7321	Functional Grade* Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	NU
17-7321	APC anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	660	IC Flow
19-7321	Cy5 anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	670	IC Flow
51-7321	Alexa Fluor® 647 anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	668	IC Flow
53-7321	Alexa Fluor® 488 anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	519	IC Flow
57-7321	Pacific Blue® anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.   Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF-α), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4⁺ T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. This MP6-XT22 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunohistology staining of frozen tissue sections. (Fluorochrome conjugated MP6-XT22 is recommended for use in intracellular flow cytometry.)

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Applications Tested

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The MP6-XT22 antibody has been tested to block staining with fluorochrome conjugated MP6-XT22. This can be used at less than or equal to 2 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Hunter CA, Litton MJ, et al. 1994. Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis. J Infect Dis. 170(4): 939-45.

Litton MJ, Sander B, et al. 1994. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J Immunol Methods 175(1): 47-58.

Abrams JS, Roncarolo MG, et al. 1992. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 127: 5-24.

Chackerian B, Lowy DR and Schiller JT. 2001. Conjugation of a self-antigen to papillomavirus-like particles allows for efficient induction of protective autoantibodies. J Clin Invest. 108(3):415-23. (IHC frozen, PubMed)

Williams RO, Mauri C, et al. 1998. Therapeutic actions of cyclosporine and anti-tumor necrosis factor alpha in collagen-induced arthritis and the effect of combination therapy. Arthritis Rheum. 41(10):1806-12. (IHC frozen, PubMed)

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