*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The NIB42 antibody reacts with human interferon-gamma (IFN-γ). The NIB42 antibody is a neutralizing antibody. IFN-γ is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-γ in synergy with other cytokines, such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The NIB42 antibody has been reported for use in capture of human IFN-γ by ELISA, and for neutralizing IFN-γ bioactivity. Use Functional Grade purified for functional assays cat.16-7318).
Applications Tested
The NIB42 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interferon-gamma (IFN-g) in combination with the biotin 4S.B3 (13-7319) antibody for detection and recombinant human IFNg (39-8319) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-8 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate. For ELISPOT Capture, the alternative clone MD-1 is recommended.
References
Meager, A., S. Parti, et al. (1984). "Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon." J Interferon Res 4(4): 619-25.
