Product Information

Contents: Affinity Purified anti-human Interferon-gamma (IFN-γ)
Catalog Number: 14-7317
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MD-1
Isotype: Mouse IgG1, κ

Human PBMCs were activated with PMA/Ionomycin in human IFN-γ ELISPOT assay. Control well is medium alone.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7317	Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA cap ELISPOT cap FA IC Flow IHC NU WB
16-7317	Functional Grade* Purified anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISPOT cap
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MD-1 antibody reacts with human, rhesus, cynomolgus and baboon interferon-gamma (IFN-γ), the signature Th1 cytokine, which is produced mainly by activated T cells, NK cells and B cells. IFN-γ is a dimeric protein. IFN-γ has antiviral and antiparasitic activities and also inhibits the proliferation of normal and transformed cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MD-1 antibody has been reported for use in intracellular flow cytometric analysis, cytokine neutralization, immunohistochemical staining, ELISA, and ELISPOT. (Fluorochrome conjugated MD-1 is recommended for use in intracellular flow cytometry.) (Please use Functional Grade purified MD-1, cat. 16-7317, in functional assays.)

Applications Tested

The MD-1 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interferon-γ (IFN-γ) in combination with the biotinylated 4S.B3 (13-7319) antibody for detection and recombinant human IFN-γ (14-8319) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.
The FG Purified format of the MD-1 antibody has been tested as the capture antibody in an ELISPOT assay (in conjunction with the biotinylated 4S.B3 antibody) and for neutralization of IFN-γ biological activity. A suitable range of concentrations of this antibody for ELISPOT capture is 1-4 µg/ml.
For in vitro neutralization, the MD-1 antibody at 30 ug/ml has been found to neutralize by 50% the effect of 1 ng/ml human IFN-γ. A more potent neutralizing antibody is clone NIB42.

References

Van der Meide, P.H., et al. 1985. J. Immuno. Meth. 79: 293.
Yazdanbakhsh, M., et al. 1993. Eur. J. Immuno. 23: 3312.
Besouw, N.M., et al. 1994. J. Med. Primatol. 23: 42.
Van der Meide, P.H., et al. 1995. J. Med. Primatol. 24: 271.
Hilkens, C. M. U., et al. 1996. J. Immunol. 156: 1722.

Related Products

Cat. 00-0000    eBioscience 10X ELISA Coating Buffer
Cat. 44-2404    Nunc MaxiSorp™ flat-bottom 96 well plate
Cat. 18-4100    Avidin Horse Radish Peroxidase (AV-HRP)
Cat. 00-4202    eBioscience ELISA Diluent Solution, 5X
Cat. 00-4203    Super AquaBlue ELISA Substrate
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control
Cat. 88-7316    Human IFNg (Interferon-gamma, IFN-g) ELISA Ready-SET-Go!
Cat. 14-7318    Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g) (clone NIB42)
Cat. 16-7318    Functional Grade Purified anti-human IFNg (Interferon-gamma, IFN-g) (clone NIB42)
Cat. 11-7319    FITC anti-human IFNg (Interferon-gamma, IFN-g) (clone 4S.B3)
Cat. 12-7319    PE anti-human IFNg (Interferon-gamma, IFN-g) (clone 4S.B3)
Cat. 13-7319    Biotin anti-human IFNg (Interferon-gamma, IFN-g) (clone 4S.B3)
Cat. 14-7319    Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g) (clone 4S.B3)
Cat. 17-7319    APC anti-human IFNg (Interferon-gamma, IFN-g) (clone 4S.B3)
Cat. 14-8319    Recombinant Human IFNg (Interferon-gamma, IFN-g)