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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe MD-1 antibody reacts with human, rhesus, cynomolgus and baboon interferon-gamma (IFN-γ), the signature Th1 cytokine, which is produced mainly by activated T cells, NK cells and B cells. IFN-γ is a dimeric protein. IFN-γ has antiviral and antiparasitic activities and also inhibits the proliferation of normal and transformed cells. Applications ReportedFor research use only, not for diagnostic or therapeutic use. The MD-1 antibody has been reported for use in intracellular flow cytometric analysis, cytokine neutralization, immunohistochemical staining, ELISA, and ELISPOT. (Fluorochrome conjugated MD-1 is recommended for use in intracellular flow cytometry.) (Please use Functional Grade purified MD-1, cat. 16-7317, in functional assays.) Applications TestedThe MD-1 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interferon-γ (IFN-γ) in combination with the biotinylated 4S.B3 (13-7319) antibody for detection and recombinant human IFN-γ (39-8319) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate. The FG Purified format of the MD-1 antibody has been tested as the capture antibody in an ELISPOT assay (in conjunction with the biotinylated 4S.B3 antibody) and for neutralization of IFN-γ biological activity. A suitable range of concentrations of this antibody for ELISPOT capture is 1-4 µg/ml. For in vitro neutralization, the MD-1 antibody at 30 ug/ml has been found to neutralize by 50% the effect of 1 ng/ml human IFN-γ. A more potent neutralizing antibody is clone NIB42. References
Gong G, Shao L, Wang Y, Chen CY, Huang D, Yao S, Zhan X, Sicard H, Wang R, Chen ZW. Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection. Blood. 2009 Jan 22;113(4):837-45. (MD-1, cross to cynomolgus, NU, PubMed) Jurado JO, Alvarez IB, Pasquinelli V, Martínez GJ, Quiroga MF, Abbate E, Musella RM, Chuluyan HE, García VE. Programmed death (PD)-1:PD-ligand 1/PD-ligand 2 pathway inhibits T cell effector functions during human tuberculosis. J Immunol. 2008 Jul 1;181(1):116-25. (MD-1, FA, PubMed) van der Meide PH, Groenestein RJ, de Labie MC, Heeney J, Pala P, Slaoui M. Enumeration of lymphokine-secreting cells as a quantitative measure for cellular immune responses in rhesus macaques. J Med Primatol. 1995 Dec;24(4):271-81. Wassenaar A, Reinhardus C, Thepen T, Abraham-Inpijn L, Kievits F. Cloning, characterization, and antigen specificity of T-lymphocyte subsets extracted from gingival tissue of chronic adult periodontitis patients. Infect Immun. 1995 Jun;63(6):2147-53. (MD-1, IHC frozen) van Besouw NM, van der Meide PH, Bakker NP. The mitogen-induced generation of interferon-gamma producing cells in cultures of rhesus monkey peripheral blood mononuclear cells is age-dependent. J Med Primatol. 1994 Jan;23(1):42-8. Yazdanbakhsh M, Sartono E, Kruize YC, Kurniawan A, van der Pouw-Kraan T, van der Meide PH, Selkirk ME, Partono F, Hintzen RQ, van Lier RA, et al. Elevated levels of T cell activation antigen CD27 and increased interleukin-4 production in human lymphatic filariasis. Eur J Immunol. 1993 Dec;23(12):3312-7. Van der Meide PH, Dubbeld M, Schellekens H. Monoclonal antibodies to human immune interferon and their use in a sensitive solid-phase ELISA. J Immunol Methods. 1985 May 23;79(2):293-305. Related ProductsCat. 00-0000 eBioscience 10X ELISA Coating Buffer Cat. 44-2404 Nunc MaxiSorp™ flat-bottom 96 well plate Cat. 18-4100 Avidin Horse Radish Peroxidase (AV-HRP) Cat. 00-4202 eBioscience ELISA Diluent Solution, 5X Cat. 00-4203 Super AquaBlue ELISA Substrate Cat. 14-4714 Affinity Purified Mouse IgG1, K Isotype Control Cat. 88-7316 Human IFNg (Interferon-gamma, IFN-g) ELISA Ready-SET-Go! Cat. 14-7318 Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g) (clone NIB42) Cat. 16-7318 Functional Grade Purified anti-human IFNg (Interferon-gamma, IFN-g) (clone NIB42) Cat. 11-7319 FITC anti-human IFNg (Interferon-gamma, IFN-g) (clone 4S.B3) Cat. 12-7319 PE anti-human IFNg (Interferon-gamma, IFN-g) (clone 4S.B3) Cat. 13-7319 Biotin anti-human IFNg (Interferon-gamma, IFN-g) (clone 4S.B3) Cat. 14-7319 Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g) (clone 4S.B3) Cat. 17-7319 APC anti-human IFNg (Interferon-gamma, IFN-g) (clone 4S.B3) Cat. 14-8319 Recombinant Human IFNg (Interferon-gamma, IFN-g) |
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Copyright © 2000-2009 eBioscience, Inc. For Research Use Only. Not for use in diagnostic procedures. Not for further distribution without written consent. |