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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe AN-18 antibody reacts with mouse interferon-gamma (IFN-γ), a 15-20 kDa cytokine secreted by Th1 cells. Applications ReportedFor research use only, not for diagnostic or therapeutic use. The AN-18 antibody has been reported for use in immunoprecipitation, ELISPOT, and ELISA. (Please use Functional Grade purified AN-18, cat. 16-7313, for ELISPOT Capture.) Applications TestedThe AN-18 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IFNg (Interferon-gamma, IFN-g) in combination with the biotin R4-6A2 (13-7312) antibody for detection and recombinant mouse IFNg (14-8311) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25-1 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 200 pg/ml - 1.6 pg/ml should be included in each ELISA plate. References
Frasca D, Adorini L, Landolfo S, Doria G. 1988. Enhancement of suppressor T cell activity by injection of anti-IFN-gamma monoclonal antibody. J Immunol. 140: 4103-7. Simon MM, Hochgeschwender U, Brugger U, Landolfo S. 1986. Monoclonal antibodies to interferon-gamma inhibit interleukin 2-dependent induction of growth and maturation in lectin/antigen-reactive cytolytic T lymphocyte precursors. J Immunol. 136: :2755-62. Landolfo S, Cofano F, Gandino L, Gribaudo G, Prat M. 1985. Monoclonal antibodies against mouse gamma-interferon inhibit tumoricidal macrophage activation by T lymphocytes. Exp Cell Biol. 53: 265-9. Prat M, Gribaudo G, Comoglio PM, Cavallo G, Landolfo S. 1984. Monoclonal antibodies against murine gamma interferon. Proc Natl Acad Sci U S A. 81: 4515-9. Slade, S., et al. 1989. Production of interferon-gamma during infection of mice with Plasmodium chabaudi. Immunobiology. 179: 353. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1. Kort JJ, Kawamura K, et al. 2006. Efficient presentation of myelin oligodendrocyte glycoprotein peptides but not protein by astrocytes from HLA-DR2 and HLA-DR4 transgenic mice. J Neuroimmunol. 173(1-2):23-34. (ELISPOT, PubMed) Related ProductsCat. 00-0000 eBioscience 10X ELISA Coating Buffer Cat. 44-2404 Nunc MaxiSorp™ flat-bottom 96 well plate Cat. 18-4100 Avidin Horse Radish Peroxidase (AV-HRP) Cat. 00-4202 eBioscience ELISA Diluent Solution, 5X Cat. 00-4203 Super AquaBlue ELISA Substrate Cat. 14-4301 Affinity Purified Rat IgG1 Isotype Control Cat. 88-7234 Mouse IL-23 (p19/p40, IL23) ELISA Ready-SET-Go! Cat. 11-7311 FITC anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2) Cat. 12-7311 PE anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2) Cat. 13-7311 Biotin anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2) Cat. 14-7311 Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2) Cat. 16-7311 Functional Grade Purified anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2) Cat. 17-7311 APC anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2) Cat. 19-7311 Cy5 anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2) Cat. 13-7312 Biotin anti-mouse IFNg (Interferon-gamma, IFN-g) (clone R4-6A2) Cat. 14-7312 Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g) (clone R4-6A2) Cat. 16-7312 Functional Grade Purified anti-mouse IFNg (Interferon-gamma, IFN-g) (clone R4-6A2) Cat. 14-8231 Recombinant Mouse IL-23 (Interleukin-23, IL23) |
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