Splenocytes from mice pre-immunized with PLP139-151 peptide were recall activated 4 weeks later with PLP139-151 for 24 hours in Mouse IFNλ ELISPOT assay. Control well is medium alone.
Contents: Anti-Mouse IFN gamma Purified Catalog Number: 14-7313 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Clone: AN-18 Host/Isotype: Rat IgG1, κ
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14-7313-81
50 ug
14-7313-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The AN-18 antibody reacts with mouse interferon-gamma (IFN-γ), a 15-20 kDa cytokine secreted by Th1 cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The AN-18 antibody has been reported for use in immunoprecipitation, ELISPOT, and ELISA. (Please use Functional Grade purified AN-18, cat. 16-7313, for ELISPOT Capture.)
Applications Tested
The AN-18 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IFNg (Interferon-gamma, IFN-g) in combination with the biotin R4-6A2 (13-7312) antibody for detection and recombinant mouse IFNg (39-8311) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25-1 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 200 pg/ml - 1.6 pg/ml should be included in each ELISA plate.
References
Frasca D, Adorini L, Landolfo S, Doria G. 1988. Enhancement of suppressor T cell activity by injection of anti-IFN-gamma monoclonal antibody. J Immunol. 140: 4103-7.
Simon MM, Hochgeschwender U, Brugger U, Landolfo S. 1986. Monoclonal antibodies to interferon-gamma inhibit interleukin 2-dependent induction of growth and maturation in lectin/antigen-reactive cytolytic T lymphocyte precursors. J Immunol. 136: :2755-62.
Landolfo S, Cofano F, Gandino L, Gribaudo G, Prat M. 1985. Monoclonal antibodies against mouse gamma-interferon inhibit tumoricidal macrophage activation by T lymphocytes. Exp Cell Biol. 53: 265-9.
Prat M, Gribaudo G, Comoglio PM, Cavallo G, Landolfo S. 1984. Monoclonal antibodies against murine gamma interferon. Proc Natl Acad Sci U S A. 81: 4515-9.
Slade, S., et al. 1989. Production of interferon-gamma during infection of mice with Plasmodium chabaudi. Immunobiology. 179: 353.
Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.
Kort JJ, Kawamura K, et al. 2006. Efficient presentation of myelin oligodendrocyte glycoprotein peptides but not protein by astrocytes from HLA-DR2 and HLA-DR4 transgenic mice. J Neuroimmunol. 173(1-2):23-34. (ELISPOT, PubMed)
